LOCUS       sequence-62386-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Ju JH, Maeng JS, Lee DY, Piszczek G, Gelmann EP, Gruschus JM
  TITLE     Interactions of the acidic domain and SRF interacting motifs with 
            the NKX3.1 homeodomain.
  JOURNAL   Biochemistry. 2009 Nov 10;48(44):10601-7. doi: 10.1021/bi9013374.
  PUBMED    19780584
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochemistry"; date: "2009-11-10- 10"; volume: "48"; issue: "44"; 
            pages: "10601-7"
COMMENT     Sequence Label: His-NKX3.1
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    73..97
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             112..134
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             145..162
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             172..189
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             196..240
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             256..270
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 ggccacgatg cgtccggcgt agaggatcga gatcgatctc gatcccgcga aattaatacg
       61 actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa
      121 ctttaagaag gagatataca tatgcaccat catcatcatc attcttctgg tctggtgcca
      181 cgcggttctg gtatgaaaga aaccgctgct gctaaattcg aacgccagca catggacagc
      241 ccagatctgg gtaccgacga cgacgacaag gccatggcta tgctcagggt tccggagccg
      301 cggcccgggg aggcgaaagc ggagggggcc gcgccgccga ccccgtccaa gccgctcacg
      361 tccttcctca tccaggacat cctgcgggac ggcgcgcagc ggcaaggcgg ccgcacgagc
      421 agccagagac agcgcgaccc ggagccggag ccagagccag agccagaggg aggacgcagc
      481 cgcgccgggg cgcagaacga ccagctgagc accgggcccc gcgccgcgcc ggaggaggcc
      541 gagacgctgg cagagaccga gccagaaagg cacttggggt cttatctgtt ggactctgaa
      601 aacacttcag gcgcccttcc aaggcttccc caaaccccta agcagccgca gaagcgctcc
      661 cgagctgcct tctcccacac tcaggtgatc gagttggaga ggaagttcag ccatcagaag
      721 tacctgtcgg cccctgaacg ggcccacctg gccaagaacc tcaagctcac ggagacccaa
      781 gtgaagatat ggtt
//
