LOCUS       sequence-62395-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F
  TITLE     A transcription activator-like effector toolbox for genome 
            engineering.
  JOURNAL   Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431.
  PUBMED    22222791
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2011"; journalName: "Nat Protoc"; date: "2012-01-5- 
            5"; volume: "7"; issue: "1"; pages: "171-92"
COMMENT     Sequence Label: pTALEN_v2 (HD)
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             384..689
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     568..587
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta
       61 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
      121 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
      181 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
      241 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
      301 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
      361 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
      421 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
      481 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
      541 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
      601 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
      661 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
      721 caggtcgacg gtctcgactc acgcctgagc aggtagtggc tattgcatcc catgacgggg
      781 gcagacccgc actggagtca atcgtggccc agctttcgag gccggacccc gcgctggccg
      841 cactcactaa tgatcatctt gtagcgctgg cctgcctcgg cggacgaccc gccttggatg
      901 cggtga
//
