LOCUS       sequence-62410-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Shen RR, Zhou AY, Kim E, Lim E, Habelhah H, Hahn WC
  TITLE     IkappaB kinase epsilon phosphorylates TRAF2 to promote mammary 
            epithelial cell transformation.
  JOURNAL   Mol Cell Biol. 2012 Dec;32(23):4756-68. doi: 10.1128/MCB.00468-12. 
            Epub 2012 Sep 24.
  PUBMED    23007157
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00468-12"; journalName: "Mol Cell Biol"; date: 
            "2012-12"; volume: "32"; issue: "23"; pages: "4756-68"
COMMENT     Sequence Label: pLKO-shcIAP1-B
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    135..252
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        301..811
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     730..748
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(833..852)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tttcttggct ttatatatct tgtggaagga cgaaacaccg ggctgcggcc aacatcttca
       61 aactcgagtt tgaagatgtt ggccgcagct ttttaattct cgacctcgag acaaatggca
      121 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
      181 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
      241 attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcc gcggctcgag
      301 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
      361 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
      421 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
      481 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
      541 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
      601 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
      661 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
      721 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
      781 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggagctta ccatgaccga
      841 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
      901 cgccgccgcg ttcgccgact accccgccac gcgccacacc
//
