LOCUS       sequence-62416-e         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Pertz O, Hodgson L, Klemke RL, Hahn KM
  TITLE     Spatiotemporal dynamics of RhoA activity in migrating cells.
  JOURNAL   Nature. 2006 Apr 20. 440(7087):1069-72.
  PUBMED    16547516
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2006 Apr 20. 440(7087):1069-72."
COMMENT     Sequence Label: pTriEx-RhoA FLARE.sc Biosensor WT
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..28
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9..27
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    28..52
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        68..177
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             198..215
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(480..482,483..485,486..944)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITA"
     primer_bind     complement(525..546)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(786..805)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 accgaaatta atacgactca ctatagggga attgtgagcg gataacaatt ccccggagtt
       61 aatccgggac ctttaattca acccaacaca atatattata gttaaataag aattattatc
      121 aaatcatttg tatattaatt aaaatactat actgtaaatt acattttatt tacaatcaaa
      181 ggagatatac catggcacac catcaccacc atcacggtag tggcatcctg gaggacctca
      241 atatgctcta catccggcag atggcactca gcctggagga cacagagctg cagaggaaac
      301 tagatcatga gatccggatg agggatgggg cctgcaagct gctggcagcc tgctcccagc
      361 gagagcaggc tctggaagcc accaagagcc tgctggtgtg caacagccgt attctcagct
      421 acatgggtga gctgcagcgg cgaaaggagg cccaggtgct ggagaagaca ggcgggggaa
      481 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      541 gcgacgtaaa cggccacagg ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      601 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      661 tcgtgaccac cctgacctgg ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
      721 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgt accatcttct
      781 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
      841 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
      901 agctggagta caactacatc agccacaacg tctatatcac cgccgacaag cagaagaacg
      961 gcatc
//
