LOCUS       sequence-62418-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV zinc finger donor vector for genomic targeting.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Hockemeyer D, Soldner F, Beard C, Gao Q, Mitalipova M, Dekelver RC, 
            Katibah GE, Amora R, Boydston EA, Zeitler B, Meng X, Miller JC, 
            Zhang L, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R
  TITLE     Efficient targeting of expressed and silent genes in human ESCs and 
            iPSCs using zinc-finger nucleases.
  JOURNAL   Nat Biotechnol. 2009 Sep;27(9):851-7. doi: 10.1038/nbt.1562
  PUBMED    19680244
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2009-09"; volume: "27"; issue:
            "9"; pages: "851-7"
COMMENT     Sequence Label: AAV-CAGGS-EGFP
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(2..4,5..7,8..721)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2..4,5..7,8..718)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(47..68)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(308..327)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     655..676
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
       61 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      121 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      181 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
      241 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      301 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      361 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      421 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
      481 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
      541 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
      601 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
      661 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
      721 aacgcgtgaa ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc
      781 caatgccctg gctcacaaat accactgaga tctttttccc
//
