LOCUS       sequence-62419-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for shRNA expression under H1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR/pSUPER+ (575-1)
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(798..814)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(799..815)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 ttgggaatct tataagttct gtatgagacc acagatctcg gcgttgtgca tgtcgggcga
       61 ctgctccatg atcttgcgcc gctcgatctg cgaccacacc ataaaggcgt tcatgggccg
      121 cttgatgtgg ccactgggcg tcttgcacca gctggggtcg tccgccttgc cgcccgtcga
      181 cgcggtggag ccaggcgtcg gggaggacgc gatgcccagc tccaggccgg cgcccgagtc
      241 cgagctctcc ccggccagca gagcctcagt gttctccgcg ttgttggtct gttgtaccat
      301 ggccccggct tgccgggcgc ccacgcgcgc tcacacgctc gcggcgcggt cgccgggccc
      361 tgcgggctcc ccggggcgga gaccagggtc ccctgagcgg cgcgcgggcc aggggcggcc
      421 gcctcccgcc gccaacctag agtccccgag gccctccgtc ctccctcacc gctgaagtgc
      481 tccctgcggg caccgaggct ggagtcggcc gcggcgagga gtttctccaa acgttggtag
      541 ccggagtatc ttccaaacgc aagtttctcg ccgcctccgg ggcaagtctt tcccccaccc
      601 ctttccctga agcagtcgat tgcagttcac gagagctcgc ggtagctcag gaaagcgaca
      661 tcgtctctag cgcctaagtc cctttcctgc agtgcaaagc caaagcgact ctggctccgg
      721 gactctctgt gggcggaatc ggcgctgagg agttggtgca attattttgt tgcaaggtag
      781 gaagccaaga agcttatcga taccgtcgac ctcgagatat ctagacccag ctttcttgta
//
