LOCUS       sequence-62425-e         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mKalama1 blue fluorescent protein with NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Ai HW, Shaner NC, Cheng Z, Tsien RY, Campbell RE
  TITLE     Exploration of New Chromophore Structures Leads to the 
            Identification of Improved Blue Fluorescent Proteins.
  JOURNAL   Biochemistry. 2007 May 22;46(20):5904-10. doi: 10.1021/bi700199g
  PUBMED    17444659
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochemistry"; date: "2007-05-22- 22"; volume: "46"; issue: "20"; 
            pages: "5904-10"
COMMENT     Sequence Label: pmKalama1-Nuc
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      48..57
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc accatggtga
       61 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagatg gacggcgacg
      121 taaacggccg caagttcagc gtgcgcggcg tgggcgaggg tgatgccacc cacggcaagc
      181 tgaccctgaa gttcatctgc acctccggca agctgcccgt gccctggcct accctcgtga
      241 ccaccctgtc ttacggcgtg cagtgcttct cccgctaccc cgaccacatg aagcagcacg
      301 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
      361 acgacggcag ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
      421 gcatcgtgct gaagggcacc gacttcaagg aggacggtaa catcctgggg cacaagctgg
      481 agtacaacat gaacgtcggg aacgtctata tcacggccga caagcagaag aacggcatca
      541 aagcgaactt cgagaccagg cacaacgtcg aggacggcgg cgtgcagctc gccgaccact
      601 accagcagaa cacccccatc ggcgacggct ccgtgctgct gcccgacaac cactacctga
      661 gcgtgcaggt gaagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
      721 agttccgcac cgccgccggg atcactcccg gcatggacga gct
//
