LOCUS       sequence-62430-e         514 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mKalama1 blue fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Ai HW, Shaner NC, Cheng Z, Tsien RY, Campbell RE
  TITLE     Exploration of New Chromophore Structures Leads to the 
            Identification of Improved Blue Fluorescent Proteins.
  JOURNAL   Biochemistry. 2007 May 22;46(20):5904-10. doi: 10.1021/bi700199g
  PUBMED    17444659
REFERENCE   2  (bases 1 to 514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochemistry"; date: "2007-05-22- 22"; volume: "46"; issue: "20"; 
            pages: "5904-10"
COMMENT     Sequence Label: pBad-mKalama1
FEATURES             Location/Qualifiers
     source          1..514
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             66..88
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             108..125
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             129..161
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             174..194
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cctgacgctt tttatcgcaa ctctctactg tttctccata cccgtttttt tgggctagaa
       61 ataattttgt ttaactttaa gaaggagata tacatatgcg gggttctcat catcatcatc
      121 atcatggtat ggctagcatg actggtggac agcaaatggg tcgggatctg tacgagaacc
      181 tgtacttcca gggctcgagc atggtgagca agggcgagga gctgttcacc ggggtggtgc
      241 ccatcctggt cgagatggac ggcgacgtaa acggccgcaa gttcagcgtg cgcggcgtgg
      301 gcgagggtga tgccacccac ggcaagctga ccctgaagtt catctgcacc tccggcaagc
      361 tgcccgtgcc ctggcctacc ctcgtgacca ccctgtctta cggcgtgcag tgcttctccc
      421 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      481 tccaggagcg caccatcttc ttcaaggacg acgg
//
