LOCUS       sequence-62435-e        1122 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Wu K, Lu G, Sehnke P, Ferl RJ
  TITLE     The heterologous interactions among plant 14-3-3 proteins and 
            identification of regions that are important for dimerization.
  JOURNAL   Arch Biochem Biophys. 1997 Mar 1;339(1):2-8.
  PUBMED    9056226
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Biochem Biophys."; date: "1997-03-1"; volume: "339(1)"; pages: "2-8"
COMMENT     Sequence Label: pET15b-At 14-3-3 psi
FEATURES             Location/Qualifiers
     source          1..1122
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atgtcgacaa gggaagagaa tgtttacatg gcgaaattag ccgaacaagc tgaacgttac
      121 gaagaaatgg ttgaattcat ggagaaagtt gcgaaaactg ttgatgttga ggaactttca
      181 gttgaagaga ggaatcttct ctctgttgct tacaagaacg tgattggagc gagaagagct
      241 tcgtggagaa tcatttcttc gattgagcag aaagaagaga gcaaagggaa cgaagatcat
      301 gttgctatta tcaaggatta cagaggaaag attgaatccg agcttagcaa aatctgtgat
      361 gggattttga atgttcttga agctcatctt attccttctg cttcaccagc tgaatctaaa
      421 gtgttttatc ttaagatgaa gggtgattat cataggtatc ttgctgagtt taaggctggt
      481 gctgaaagga aagaagctgc tgaaagcact ttggttgctt acaagtctgc ttccgacatt
      541 gccactgctg agttagctcc tactcacccg ataaggcttg gtcttgcact caacttctct
      601 gtgttttact atgaaatcct caactcgcct gatcgtgctt gcagcctcgc aaagcaggcg
      661 tttgatgatg caatcgctga gttagataca ttgggtgagg aatcatacaa ggacagtaca
      721 ctgattatgc agcttcttag agacaatctc actctctgga cttcagatat gactgacgaa
      781 gcaggagatg agattaagga ggcatcaaag cccgatggtg ccgagtaaac ccgaagaaga
      841 ggaagagtca ttttataatg gtagaaaact ttcgtgtgat aatctgagtg agggatagct
      901 caggattctt ataagagaca cagtttttat tgtgtttggt ttgttttgaa actacttgcg
      961 attatcattc ctcctttagc ttctcaatat ttcctattct cctttctaat gaatcaaact
     1021 gtaaaaaaaa ccgatttcag ctgagcgcgg tcgctaccgg atcngctgct taacaaancc
     1081 ggaagangct gaggtttggg cttggcgtgg cccacacccg cg
//
