LOCUS       sequence-62445-e         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Stellberger T, Hauser R, Baiker A, Pothineni VR, Haas J, Uetz P
  TITLE     Improving the yeast two-hybrid system with permutated fusions 
            proteins: the Varicella Zoster Virus interactome.
  JOURNAL   Proteome Sci. 2010 Feb 15;8:8. doi: 10.1186/1477-5956-8-8.
  PUBMED    20205919
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteome 
            Sci."; date: "2010-02-15"; volume: "8:8. doi"; pages: " 
            10.1186/1477-5956-8-8"
COMMENT     Sequence Label: pGADCg
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    49..173
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(214..519)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(316..335)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gctatcagca tattcccact ttgattaaaa ttggcggcgg taccatcaac cactttgtac
       61 aagaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
      121 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atggtcgacc
      181 tgcagactgg ctgtgtataa gggagcctga catttatatt ccccagaaca tcaggttaat
      241 ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga
      301 gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac
      361 caccgggtaa agttcacggg agactttatc tgacagcaga cgtgcactgg ccagggggat
      421 caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa acagacgata
      481 acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagccc ctgttctcgt
      541 cagcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct tcctgatttt
      601 ccgctttcca gcgttcggca cgcagacgac gggcttcatt ctgcatggtt gtgcttacca
      661 gaccggagat attgacatca tatatgcctt gagcaactga tagctgtcgc tgtcaactgt
      721 cactgtaata cgctgcttca tagcatacct ctttttgaca tactc
//
