LOCUS       sequence-62447-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Stellberger T, Hauser R, Baiker A, Pothineni VR, Haas J, Uetz P
  TITLE     Improving the yeast two-hybrid system with permutated fusions 
            proteins: the Varicella Zoster Virus interactome.
  JOURNAL   Proteome Sci. 2010 Feb 15;8:8. doi: 10.1186/1477-5956-8-8.
  PUBMED    20205919
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteome 
            Sci."; date: "2010-02-15"; volume: "8:8. doi"; pages: " 
            10.1186/1477-5956-8-8"
COMMENT     Sequence Label: pGADCg
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             181..201
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    220..344
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(369..374,375..392,393..399)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(519..538)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ttgctgtctt gctatcaagt ataaatagac ctgcaattat taatcttttg tttcctcgtc
       61 attgttctcg ttccctttct tccttgtttc tttttctgca caatatttca agctatacca
      121 agcatacaat caactccaag ctttgcaaag atggataaag cggaattaat tcccgagcct
      181 ccaaaaaaga agagaaaggt cgaattgggt accgcatcaa caagtttgta caaaaaagct
      241 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
      301 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca
      361 ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc
      421 cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
      481 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
      541 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
      601 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
      661 atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
      721 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc
      781 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
      841 acctggccta tttccctaaa ggg
//
