LOCUS       sequence-62448-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  TITLE     Nematode antibiotic selection (G418)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRG02
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(197..301)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      328..784
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(415..434)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     625..646
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
       61 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
      121 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
      181 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
      241 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
      301 gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg
      361 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      421 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
      481 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
      541 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
      601 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
      661 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
      721 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
      781 atttccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgg
//
