LOCUS       sequence-62449-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Blelloch R, Venere M, Yen J, Ramalho-Santos M
  TITLE     Generation of induced pluripotent stem cells in the absence of drug 
            selection.
  JOURNAL   Cell Stem Cell. 2007 Sep 13;1(3):245-7. doi: 
            10.1016/j.stem.2007.08.008. Epub 2007 Sep 6.
  PUBMED    18371358
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2007.08.008"; journalName: "Cell Stem Cell"; date: 
            "2007-09-13- 13"; volume: "1"; issue: "3"; pages: "245-7"
COMMENT     Sequence Label: pLOVE empty vector
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    80..113
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(151..192)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    245..369
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(410..715)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(512..531)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattgta cctagtggaa
       61 ccggaaccct taaacatgta taacttcgta taatgtatgc tatacgaagt tattaggtcc
      121 ctcgacgaat tgtcattact aaccggtacg cgtagaatcg agaccgagga gagggttagg
      181 gataggctta ccttcgaacc gcgggccctc tagactcgag cggccgccac tgtgctggat
      241 atcaaccact ttgtacaaga aagctgaacg agaaacgtaa aatgatataa atatcaatat
      301 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
      361 gtcactatgg tcgacctgca gactggctgt gtataaggga gcctgacatt tatattcccc
      421 agaacatcag gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt
      481 cttccccgat aacggagacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt
      541 catccccgat atgcaccacc gggtaaagtt cacgggagac tttatctgac agcagacgtg
      601 cactggccag ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat
      661 ccacaaacag acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac
      721 cagcccctgt tctcgtcagc aaaagagccg ttcatttcaa taaaccgggc gacctcagcc
      781 atcccttcct gattttccgc tttccagcgt tcggc
//
