LOCUS       sequence-62450-e         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Livingston CM, Ifrim MF, Cowan AE, Weller SK
  TITLE     Virus-Induced Chaperone-Enriched (VICE) domains function as nuclear 
            protein quality control centers during HSV-1 infection.
  JOURNAL   PLoS Pathog. 2009 Oct . 5(10):e1000619.
  PUBMED    19816571
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog. 2009 Oct . 5(10):e1000619."
COMMENT     Sequence Label: pRK5-HA-Ubiquitin-K27
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             111..137
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             168..395
                     /codon_start=1
                     /product="human ubiquitin variant containing Lys-27, with 
                     all other lysines mutated to arginine"
                     /label=ubiquitin-K27
                     /translation="MQIFVRTLTGRTITLEVEPSDTIENVKARIQDREGIPPDQQRLIF
                     AGRQLEDGRTLSDYNIQRESTLHLVLRLRGG"
     regulatory      432..441
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    445..579
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(482..501)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     536..555
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     enhancer        648..839
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(671..691)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ttctctccac aggtgtccac tcccaggtcc aactgcacct cggttctatc gattgaattc
       61 ccgaaccgac agtcggtctc ttcaccaagg ccattcgcgc caccatgggc tacccctatg
      121 atgtgcctga ctacgcagat ctcaatggtg gtggtggtgg gtcgaccatg cagatcttcg
      181 tcagaacgtt aaccggtaga accataactc tagaagttga accatccgat accatcgaaa
      241 acgttaaagc tagaattcaa gacagagaag gcattccacc tgatcaacaa agattgatct
      301 ttgccggtag acagctcgag gacggtagaa cgctgtctga ttacaacatt cagagagagt
      361 cgaccttaca tcttgtctta agactaagag gtggttgagc ggccgctaag taagtaagga
      421 tccccagctt ggccgccatg gcccaacttg tttattgcag cttataatgg ttacaaataa
      481 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
      541 ttgtccaaac tcatcaatgt atcttatcat gtctggatcg ggaattaatt cggcgcagca
      601 ccatggcctg aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag
      661 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
      721 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
      781 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
      841 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
      901 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
      961 cagaagtagt gaggagg
//
