LOCUS       sequence-62451-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Lemaire PA, Lary J, Cole JL
  TITLE     Mechanism of PKR activation: dimerization and kinase activation in 
            the absence of double-stranded RNA.
  JOURNAL   J Mol Biol. 2005 Jan 7;345(1):81-90.
  PUBMED    15567412
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2005-01-7"; volume: "345(1)"; pages: "81-90"
COMMENT     Sequence Label: pPET-PKR/PPase
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..81
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    81..105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             120..142
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa
       61 ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt
      121 ttgtttaact ttaagaagga gatatacata tggctggtga tctttcagca ggtttcttca
      181 tggaggaact taatacatac cgtcagaagc agggagtagt acttaaatat caagaactgc
      241 ctaattcagg acctccacat gataggaggt ttacatttca agttataata gatggaagag
      301 aatttccaga aggtgaaggt agatcaaaga aggaagcaaa aaatgccgca gccaaattag
      361 ctgttgagat acttaataag gaaaagaagg cagttagtcc tttattattg acaacaacga
      421 attcttcaga aggattatcc atggggaact acataggcct tatcaataga attgcccaga
      481 agaaaagact aactgtaaat tatgaacagt gtgcatcggg ggtgcatggg ccagaaggat
      541 ttcattataa atgcaaaatg ggacagaaag aatatagtat tggtacaggt tctactaaac
      601 aggaagcaaa acaattggcc gctaaacttg catatcttca gatattatca gaagaaacct
      661 cagtgaaatc tgactacctg tcctctggtt cttttgctac tacgtgtgag tcccaaagca
      721 actctttagt gaccagcaca ctcgcttctg aatcatcatc tgaaggtgac ttctcagcag
      781 atacatcaga gataaattct aacagtgaca gtttaaacag ttcttcgttg cttatgaatg
      841 gtctcagaaa taatcaaagg aaggcaaaaa gatctttggc acccagattt gaccttcctg
      901 acatgaaaga aacaaagtat actgtggaca
//
