LOCUS       sequence-62454-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Orimo A, Gupta PB, Sgroi DC, Arenzana-Seisdedos F, Delaunay T, Naeem
            R, Carey VJ, Richardson AL, Weinberg RA
  TITLE     Stromal fibroblasts present in invasive human breast carcinomas 
            promote tumor growth and angiogenesis through elevated SDF-1/CXCL12 
            secretion.
  JOURNAL   Cell. 2005 May 6. 121(3):335-48.
  PUBMED    15882617
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2005 
            May 6. 121(3):335-48."
COMMENT     Sequence Label: pLKO.1 puro GFP siRNA
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    149..266
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        315..798
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     744..762
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgggcc cgcaagctga
       61 ccctgaagtt cattcaagag atgaacttca gggtcagctt gctttttgaa ttctcgacct
      121 cgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
      181 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
      241 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
      301 ggccgcggct cgagggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag
      361 ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc
      421 acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc
      481 ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc
      541 cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag
      601 ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg
      661 cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg
      721 tgggccctgt tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg
      781 gcagtcggct ccctcgttga ccgaatcacc gacctctctc c
//
