LOCUS       sequence-62457-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Nakano T, Matsushima-Hibiya Y, Yamamoto M, Takahashi-Nakaguchi A, 
            Fukuda H, Ono M, Takamura-Enya T, Kinashi H, Totsuka Y
  TITLE     ADP-ribosylation of guanosine by SCO5461 protein secreted from 
            Streptomyces coelicolor.
  JOURNAL   Toxicon. 2012 Dec 2;63C:55-63. doi: 10.1016/j.toxicon.2012.11.019.
  PUBMED    23212047
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Toxicon."; 
            date: "2012-12-2"; volume: "63C:55-63. doi"; pages: " 
            10.1016/j.toxicon.2012.11.019"
COMMENT     Sequence Label: pMALp2x-SCO5461(E164D)(43-204)
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     128..150
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     142..159
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     143..159
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(680..691)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(743..766)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 tgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
       61 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
      121 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagcttgc cactgcagtc
      181 agtgccaggg ctggtagtgc gggttgctct cgcagtcgct catgatctcg gtcttggtct
      241 gccggtcgac cggacagacg ccgatgatgt acttccgctg gatgccgccc gggaaggcga
      301 cgtcgacctg gtcggcccac ttgtgggtgt cgccgatggt cttgttgacg tcgatgccgc
      361 cgggggcgtc gacgtagtag ttgtagccgg acttgtacca ggtcttgtac aggtcgttgt
      421 cgtagctcgt cgacacgtac ggcgagggct ggttgaccag gacgtacttc tcgacgtcgt
      481 actgtccgtt ctggacgtcc ttggcgtgga agccctcctc gaagacgacc tgcgggccgc
      541 ggctgtcgct gcggtagagg gtgccgcagg tggtgcgcca gaccggctcg ggcgtgatgc
      601 ggtcgacgtc gacgccgcgg tcggcggcgg ccttggtccg gtcgtcgaac tgggggcagg
      661 ccggggcgaa ttctgaaatc cttccctcga tcccgaggtt gttgttattg ttattgttgt
      721 tgttgttcga gctcgaatta gtctgcgcgt ctttcagggc ttcatcgaca gtctgacgac
      781 cgctgggcgg cgttgatcac cgcagtacgc acggcatacc agaaaagcgg aca
//
