LOCUS       sequence-62458-e         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Chang CW, Lai YS, Pawlik KM, Liu K, Sun CW, Li C, Schoeb TR, Townes 
            TM
  TITLE     Polycistronic lentiviral vector for ""hit and run"" reprogramming of
            adult skin fibroblasts to induced pluripotent stem cells.
  JOURNAL   Stem Cells. 2009 May . 27(5):1042-9.
  PUBMED    19415770
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2009 May . 27(5):1042-9."
COMMENT     Sequence Label: pKP332 (Lenti-OSK1)
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(275..331)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 cgttgaactc ctcggtctct ctccgaggta ggggcgccag gttgctaccg ccgcaagccg
       61 caccggctcc gccgctctcc aggtctgtgg ccacggtcgc cgccgccagg tcataggggc
      121 ggccgggaag cactggggga agtcgcttca tgtgggagag ctcctcccgc cagcggttat
      181 tcggggcacc tgcttgacgc agtgtcttct cccttcccgc cgggccagac gcgaacgtgg
      241 agaaagatgg gagcagcgcg tcgctgacag ccataggccc ggggttttct tcaacatctc
      301 ctgcttgctt taacagagag aagttcgtgg ctccggatcc catgtgtgag aggggcagtg
      361 tgccgttaat ggccgtgccg ggcaccgggc cgctctggta gtgctgggac atgtgaagtc
      421 tgctgggggc ggcgggttcc ggcacctcgg cgccggggag atacatgctg atcatgtccc
      481 ggaggtcccc ggcctggcag ggcgccctgg agtgggagga agaggtaacc acaggggggc
      541 tggagctggc ctcggacttg accaccgaac ccatggagcc aagagccatg ccaggggtgc
      601 cctgctgcga gtaggacatg ctgtaggtgg gcgagccgtt catgtaggtc tgcgagctgg
      661 tcatggagtt gtactgcagg gcgctcacgt cgtagcggtg catgggctgc atctgcgctg
      721 cgccgtgcgc attgaggccc gggtgctgcg ggtagcccag ctggtcctgc atcatgctgt
      781 agctgccgtt gctccagccg ttcatgtgcg cgtaactgtc catgcgctgg ttcacgcccg
      841 cgcccaggcc ggcgcccacc ccgaccccgc tcgccatgct attgccgccg ggggccagca
      901 gcccgccggg cagcgtgtac ttatccttct tcatgagcgt cttggttttc cgccggggcc
      961 ggtatttata atccgggtgc t
//
