LOCUS       sequence-62459-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Liu JA, Wu MH, Yan CH, Chau BK, So H, Ng A, Chan A, Cheah KS, 
            Briscoe J, Cheung M
  TITLE     Phosphorylation of Sox9 is required for neural crest delamination 
            and is regulated downstream of BMP and canonical Wnt signaling.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Feb 19;110(8):2882-7. doi: 
            10.1073/pnas.1211747110. Epub 2013 Feb 4.
  PUBMED    23382206
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1211747110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-02-19- 19"; volume: "110"; issue: "8"; pages: "2882-7"
COMMENT     Sequence Label: pCIG-Flag-Sox9-IRES-nls-GFP
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             130..153
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgttatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc
       61 tcatcatttt ggcaaagaat tgctcgaggt cgatcgacgg tatcgataag cttgatagct
      121 tccaccatgg actacaagga cgacgatgac aagggatccg aattcatgaa tctcctagac
      181 cccttcatga aaatgacaga agaacaggac aaatgcatct ccgacgcccc cagccccacc
      241 atgtcggatg actccgccgg gtccccctgc ccctccggat ccggctcgga cacggagaac
      301 acccgacctc aagagaacac cttccccaaa ggcgacccgg acctgaagaa ggagagcgac
      361 gaggacaaat tccccgtgtg catccgcgag gccgtgagcc aggtgctcaa gggctacgac
      421 tggaccctgg tgcccatgcc cgtgcgggtg aacggatcca gcaagaacaa accccacgtg
      481 aagcgcccca tgaacgcctt catggtgtgg gcccaggcgg ctcgaaggaa gctggctgac
      541 cagtacccgc atctgcacaa cgccgagctc agcaagacgc tgggcaagct gtggaggctg
      601 ctgaatgaga gcgagaagcg tcccttcgtg gaggaggccg agcggctgcg ggtgcagcac
      661 aagaaggacc accccgacta caagtaccaa ccacgcagga ggaagtcggt gaagaacggg
      721 cagtcggagc aggaggaggg ctccgagcag acccacatct cccccaacgc catcttcaag
      781 gcgctgcagg
//
