LOCUS       sequence-62462-e         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Liu JA, Wu MH, Yan CH, Chau BK, So H, Ng A, Chan A, Cheah KS, 
            Briscoe J, Cheung M
  TITLE     Phosphorylation of Sox9 is required for neural crest delamination 
            and is regulated downstream of BMP and canonical Wnt signaling.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Feb 19;110(8):2882-7. doi: 
            10.1073/pnas.1211747110. Epub 2013 Feb 4.
  PUBMED    23382206
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1211747110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-02-19- 19"; volume: "110"; issue: "8"; pages: "2882-7"
COMMENT     Sequence Label: pCIG-V5-Snail2-IRES-nls-GFP
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             145..165
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             169..189
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             193..213
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(223..225,226..228,229..741)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(268..289)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(529..548)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gatctgatct ggggcctcgg tacacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
       61 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg
      121 gccacaacca tggtgcacgt ggatccaaaa aagaagagaa aggtagatcc aaaaaagaag
      181 agaaaggtag atccaaaaaa gaagagaaag gtacacgtgt ccatggtgag caagggcgag
      241 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      301 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      361 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
      421 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
      481 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
      541 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      601 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
      661 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
      721 aagatccgcc acaacatcga ggacggcagc gtgcagctcg cc
//
