LOCUS       sequence-62464-e         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Liu JA, Wu MH, Yan CH, Chau BK, So H, Ng A, Chan A, Cheah KS, 
            Briscoe J, Cheung M
  TITLE     Phosphorylation of Sox9 is required for neural crest delamination 
            and is regulated downstream of BMP and canonical Wnt signaling.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Feb 19;110(8):2882-7. doi: 
            10.1073/pnas.1211747110. Epub 2013 Feb 4.
  PUBMED    23382206
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1211747110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-02-19- 19"; volume: "110"; issue: "8"; pages: "2882-7"
COMMENT     Sequence Label: pCIG-Flag-Sox9-IRES-nls-GFP
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             153..173
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             177..197
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             201..221
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(231..233,234..236,237..695)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(276..297)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(537..556)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttgtatggga tctgatctgg ggcctcggta cacatgcttt acatgtgttt agtcgaggtt
       61 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
      121 ataatatggc cacaaccatg gtgcacgtgg atccaaaaaa gaagagaaag gtagatccaa
      181 aaaagaagag aaaggtagat ccaaaaaaga agagaaaggt acacgtgtcc atggtgagca
      241 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      301 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      361 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      421 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
      481 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      541 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
      601 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
      661 acaactacaa cagccacaac gtctatatca tggccgaca
//
