LOCUS       sequence-62465-e        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Chang CW, Lai YS, Pawlik KM, Liu K, Sun CW, Li C, Schoeb TR, Townes 
            TM
  TITLE     Polycistronic lentiviral vector for ""hit and run"" reprogramming of
            adult skin fibroblasts to induced pluripotent stem cells.
  JOURNAL   Stem Cells. 2009 May . 27(5):1042-9.
  PUBMED    19415770
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2009 May . 27(5):1042-9."
COMMENT     Sequence Label: pKP332 (Lenti-OSK1)
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          706..909
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(729..749)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ttctgctttg catatctcct gaagattttc attgttgtca gcttcctcca cccacttctg
       61 cagcaagggc cgcagcttac acatgttctt gaagctaagc tgcagagcct caaagcggca
      121 gatggtcgtt tggctgaata ccttcccaaa tagaaccccc agggtgagcc ccacatcggc
      181 ctgtgtatat cccagggtga tcctcttctg cttcaggagc ttggcaaatt gctcgagttc
      241 tttctgcaga gctttgatgt cctgggactc ctccgggttt tgctccagct tctccttctc
      301 cagcttcacg gcaccagggg tgacggtgca gggctccggg gaggccccat cggagttgct
      361 ctccaccccg actcctgctt cgccctcagg ctgagaggtc tccaagccgc cttggggcac
      421 tagccccact ccaacctggg gcccacagta cgccatcccc ccacagaact catacggcgg
      481 ggggcatggg ggaatccccc acacctcaga gcctggccca acccccggcc cgattcctgg
      541 ccctccagga gggccttgga agcttagcca ggtccgagga tcaacccagc ccggctccgg
      601 cccccctggc ccatcacctc caccacctgg agggggcgag aaggcgaaat ccgaagccag
      661 gtgtcccgcc atggtggatt taaatgtcga aattcctcac gacacctgaa atggaagaaa
      721 aaaactttga accactgtct gaggcttgag aatgaaccaa gatccaaact caaaaagggc
      781 aaattccaag gagaattaca tcaagtgcca agctggccta acttcagtct ccacccactc
      841 agtgtgggga aactccatcg cataaaaccc ctccccccaa cctaaagacg acgtactcca
      901 aaagctcgag aactaatcga ggtgcctgga cggcgcccgg tactccgtgg agtcacatga
      961 agcgacggct gaggacggaa aggccctttt cctttgtgtg ggtgactca
//
