LOCUS       sequence-62467-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Christofk HR, Vander Heiden MG, Wu N, Asara JM, Cantley LC
  TITLE     Pyruvate kinase M2 is a phosphotyrosine-binding protein.
  JOURNAL   Nature. 2008 Mar 13;452(7184):181-6. doi: 10.1038/nature06667.
  PUBMED    18337815
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2008-03-13- 13"; volume: "452"; issue: "7184"; 
            pages: "181-6"
COMMENT     Sequence Label: pET-28a-hPKM1
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattctc acggcacagg aacaacacgc atggtgttgg
      121 tgaagccgga gccagggcgc catccggtca gcacaatgac cacatctccc ttcttgaaga
      181 agcctcgggc cttgccaaca ttcatggcaa agttcacccg gaggtccacg tcctcagccc
      241 aggcctcctg gactgggtcc ttgcacagca cagggaagat gccacggtac aggtgggcct
      301 gacgagctgt ctggggattc cgggtcacag caatgatggg ggcacgtggg cggtatctgg
      361 ccacctggtg agcagacctg ccagactccg tcagaactat caaagctgct gctaaacact
      421 tataagaagc ctccacgctg cccatggcca tggcttccat gaggtctgtg gagtgacttg
      481 aggctcgcac aagttcttca aacagcttgc ggtggaacat ggctgcctca gcctcacgag
      541 ctatcaggtg ctgcatgcgc acagcctcca gaggatagtc ccctttggct gtttctccag
      601 acagcatgat gcagtcggct ccatccagga ctgcattggc cacatcactg ccttcagccc
      661 gagtggggcg gggcttcttg atcatgctct ccagcatctg agtagcacag atgacaggct
      721 tcccagctcg gttgcaccgt ccaatcatca tcttctgagc aaggaagacc ttctctgcag
      781 gaatctcaat gcctagatca ccacgagcca ccatgatccc atcactggcc tccaggattt
      841 catcaaacct ccgaaccccc tcatgattct cgattttgct gataatcttg atgttctttc
      901 ccttctctcc caggaccttc ctaacttcat ggacatc
//
