LOCUS       sequence-62469-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Christofk HR, Vander Heiden MG, Wu N, Asara JM, Cantley LC
  TITLE     Pyruvate kinase M2 is a phosphotyrosine-binding protein.
  JOURNAL   Nature. 2008 Mar 13;452(7184):181-6. doi: 10.1038/nature06667.
  PUBMED    18337815
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2008-03-13- 13"; volume: "452"; issue: "7184"; 
            pages: "181-6"
COMMENT     Sequence Label: pET-28a-hPKM2
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtcacggca
       61 caggaacaac acgcatggtg ttggtgaagc cggagccagg gcgccatccg gtcagcacaa
      121 tgaccacatc tcccttcttg aagaagcctc gggccttgcc aacattcatg gcaaagttca
      181 cccggaggtc cacgtcctca gcccaggcct cctggactgg gtccttgcac agcacaggga
      241 agatgccacg gtacaggtgg gcctgacgag ctgtctgggg attccgggtc acagcaatga
      301 tgggggcacg tgggcggtat ctggccacct ggtgagcaga cctgccagac ttggtgagga
      361 cgattatggc cccactgcag cacttgaagg aggcctccac ggcacccacg gcggtggctt
      421 ctgtggggtc gctggtaatg ggcgccaggc ggcggagttc ctcaaataat tgcaagtggt
      481 agatggcagc ctctgcctca cgggcaatca ggtgctgcat gcgcacagcc tccagaggat
      541 agtccccttt ggctgtttct ccagacagca tgatgcagtc ggctccatcc aggactgcat
      601 tggccacatc actgccttca gcccgagtgg ggcggggctt cttgatcatg ctctccagca
      661 tctgagtagc acagatgaca ggcttcccag ctcggttgca ccgtccaatc atcatcttct
      721 gagcaaggaa gaccttctct gcaggaatct caatgcctag atcaccacga gccaccatga
      781 tcccatcact ggcctccagg atttcatcaa acctccgaac cccctcatga ttctcgattt
      841 tgctgataat cttgatgttc tttcccttct ctcccaggac cttcctaact tcatggacat
      901 ca
//
