LOCUS       sequence-62473-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Blonska M, You Y, Geleziunas R, Lin X
  TITLE     Restoration of NF-kappaB activation by tumor necrosis factor alpha 
            receptor complex-targeted MEKK3 in receptor-interacting 
            protein-deficient cells.
  JOURNAL   Mol Cell Biol. 2004 Dec;24(24):10757-65.
  PUBMED    15572679
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2004-12"; volume: "24(24)"; pages: "10757-65"
COMMENT     Sequence Label: pcDNA3.1 Myc-huMEKK3 (K391A)
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             98..127
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc caccatggaa caaaaactca tctcagaaga
      121 ggatctgaag cttagatcta tggacgaaca ggaggcattg aactcaatca tgaacgatct
      181 ggtggccctc cagatgaacc gacgtcaccg gatgcctgga tatgagacca tgaagaacaa
      241 agacacaggt cactcaaata ggcagagtga cgtcagaatc aagttcgagc acaacgggga
      301 gaggcgaatt atagcgttca gccggcctgt gaaatatgaa gatgtggagc acaaggtgac
      361 aacagtattt ggacaacctc ttgatctaca ttacatgaac aatgagctct ccatcctgct
      421 gaaaaaccaa gatgatcttg ataaagcaat tgacatttta gatagaagct caagcatgaa
      481 aagccttagg atattgctgt tgtcccagga cagaaaccat aacagttcct ctccccactc
      541 tgaggtgtcc agacaggtgc ggatcaaggc ttcccagtcc gcaggggata taaatactat
      601 ctaccagccc cccgagccca gaagcaggca cctctctgtc agctcccaga accctggccg
      661 aagctcacct ccccctggct atgttcctga gcggcagcag cacattgccc ggcaggggtc
      721 ctacaccagc atcaacagtg agggggagtt catcccagag accagcgagc agtgcatgct
      781 ggatcccctg agcagtgcag aaaattcctt gtctggaagc tgccaatcct tggacaggtc
      841 agcagacagc ccatccttcc ggaaatcacg aatgtcccgt gcccagagct tccctgacaa
      901 cagacaggaa tactcagatc gggaaactca gctttatgac aaaggggtca aagg
//
