LOCUS       sequence-62476-e        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Blonska M, You Y, Geleziunas R, Lin X
  TITLE     Restoration of NF-kappaB activation by tumor necrosis factor alpha 
            receptor complex-targeted MEKK3 in receptor-interacting 
            protein-deficient cells.
  JOURNAL   Mol Cell Biol. 2004 Dec;24(24):10757-65.
  PUBMED    15572679
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2004-12"; volume: "24(24)"; pages: "10757-65"
COMMENT     Sequence Label: pcDNA Myc-mMEKK2
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      92..101
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             101..130
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgccaccatg gagcaaaagc tcatttctga
      121 agaggacttg atggatgatc agcaagcttt gaattcaatc atgcaagatt tggctgtcct
      181 tcataaggcc agtcggccag cattatcttt acaagaaacc aggaaagcaa aaccttcatc
      241 accaaaaaaa cagaatgatg ttcgagtcaa atttgaacat agaggagaaa aaaggatcct
      301 gcaggttact agaccagtta aactagaaga cctgagatct aagtctaaga tcgcctttgg
      361 gcagtctatg gatctacact ataccaacaa tgagttggta attccgttaa ctacccaaga
      421 tgacttggac aaagctgtgg aactgctgga tcgcagtatt cacatgaaga gtctcaagat
      481 attacttgta gtaaatggga gtacacaggc tactaattta gaaccatcac cgtcaccaga
      541 agatttgaat aatacaccac ttggtgcaga gaggaaaaag cggctatctg tagtaggtcc
      601 ccctaatagg gatagaagtt cccctcctcc aggatacatt ccagacgagc tacaccagat
      661 tgcccggaat gggtcattca ctagcatcaa cagtgaagga gagttcattc cagagagcat
      721 ggaccaaatg ctggatccat tgtctttaag cagccctgaa aattctggct caggaagctg
      781 tccgtcactt gatagtcctt tggatggaga aagctaccca aaatcacgga tgcctagggc
      841 acagagctac ccagataatc atcaggagtt tacagactat gataacccca tttttgagaa
      901 atttggaaaa ggaggaacat atccaagaag gtaccacgtt tcctatcatc accaggagta
      961 taatgacggt cggaagactt ttccaagagc tagaaggacc cagggc
//
