LOCUS       sequence-62479-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  used in the derivation of human iPS cells using non-integrating 
            episomal vectors; expresses Oct4 and Sox2; Myc and Klf4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA
  TITLE     Human Induced Pluripotent Stem Cells Free of Vector and Transgene 
            Sequences
  JOURNAL   Science. 2009 Mar 26.
  PUBMED    19325077
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2009 Mar 26."
COMMENT     Sequence Label: pEP4 E02S EM2K
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    487..949
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(647..666)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     876..893
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 ccgcgcccag gccggcgccc accccgaccc cgctcgccat gctattgccg ccgggggcca
       61 gcagcccgcc gggcagcgtg tacttatcct tcttcatgag cgtcttggtt ttccgccggg
      121 gccggtattt ataatccggg tgctccttca tgtgcagcgc tcgcagccgc ttagcctcgt
      181 cgatgaacgg ccgcttctcc gtctccgaca aaagtttcca ctcggcgccc aggcgcttgc
      241 tgatctccga gttgtgcatc ttggggttct cctgggccat cttgcgccgc tgcccgcggg
      301 accacaccat gaaggcattc atgggccgct tgacgcggtc cgggctgttt ttctggttgc
      361 cgccggccgc cgccgcggtg gagttgccgc cgccgccccc cgaagtttgc tgcgggcccg
      421 gcggcttcag ctccgtctcc atcatgttgt acatgcggaa tcgaattaat tcgcggtgtg
      481 gccatattat catcgtgttt ttcaaaggaa aaccacgtcc ccgtggttcg gggggcctag
      541 acgttttttt aacctcgact aaacacatgt aaagcatgtg taccgaggcc ccagatcaga
      601 tcccatacaa tggggtacct tctgggcatc cttcagcccc ttgttgaata cgcttgagga
      661 gagccatttg actctttcca caactatcca actcacaacg tggcactggg gttgtgccgc
      721 ctttgcaggt gtatcttata cacgtggctt ttggccgcag aggcacctgt cgccaggtgg
      781 ggggttccgc tgcctgcaaa gggtcgctac agacgttgtt tgtcttcaag aagcttccag
      841 aggaactgct tccttcacga cattcaacag accttgcatt cctttggcga gaggggaaag
      901 acccctagga atgctcgtca agaagacagg gccaggtttc cgggccctc
//
