LOCUS       sequence-62483-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F
  TITLE     A transcription activator-like effector toolbox for genome 
            engineering.
  JOURNAL   Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431.
  PUBMED    22222791
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2011"; journalName: "Nat Protoc"; date: "2012-01-5- 
            5"; volume: "7"; issue: "1"; pages: "171-92"
COMMENT     Sequence Label: pHD_v2
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(585..602)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(585..601)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(700..717)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gggggtgtcg cccttcgctg aactcacccc agagcaggtc gtggcaattg cgagccatga
       61 cgggggaaag caggcactcg aaaccgtcca gaggttgctg cctgtgctgt gccaagcgca
      121 cggacgtcaa aagggcgaca caaaatttat tctaaatgca taataaatac tgataacatc
      181 ttatagtttg tattatattt tgtattatcg ttgacatgta taattttgat atcaaaaact
      241 gattttccct ttattatttt cgagatttat tttcttaatt ctctttaaca aactagaaat
      301 attgtatata caaaaaatca taaataatag atgaatagtt taattatagg tgttcatcaa
      361 tcgaaaaagc aacgtatctt atttaaagtg cgttgctttt ttctcattta taaggttaaa
      421 taattctcat atatcaagca aagtgacagg cgcccttaaa tattctgaca aatgctcttt
      481 ccctaaactc cccccataaa aaaacccgcc gaagcgggtt tttacgttat ttgcggatta
      541 acgattactc gttatcagaa ccgcccaggg ggcccgagct taagactggc cgtcgtttta
      601 caacacagaa agagtttgta gaaacgcaaa aaggccatcc gtcaggggcc ttctgcttag
      661 tttgatgcct ggcagttccc tactctcgcc ttccgcttcc tcgctcactg actcgctgcg
      721 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
      781 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
      841 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
      901 tcacaaaaat cgacgctcaa gtca
//
