LOCUS       sequence-62484-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Sehnke PC, Laughner B, Cardasis H, Powell D, Ferl RJ
  TITLE     Exposed loop domains of complexed 14-3-3 proteins contribute to 
            structural diversity and functional specificity.
  JOURNAL   Plant Physiol. 2006 Feb;140(2):647-60. Epub 2006 Jan 11.
  PUBMED    16407442
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2006-02"; volume: "140(2)"; pages: "647-60. Epub 
            2006 Jan 11"
COMMENT     Sequence Label: pET15b-At 14-3-3 iota
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atgtcatcat caggatccga caaagagaga gagacttttg tctatatggc taagctctcc
      121 gaacaagccg agcgatacga cgagatggtg gagacgatga agaaagtggc gagggttaat
      181 agcgagctga ctgtggaaga gaggaatctg ttgtcggttg gttacaagaa cgtgatcgga
      241 gcaagaagag cttcgtggag gataatgtcg tccatcgagc agaaagaaga gtctaaaggc
      301 aatgaatcaa acgtgaaaca aatcaagggt tatcgccaaa aggtagaaga tgagctcgct
      361 aacatttgcc aagacattct caccatcatt gatcagcatc tcatccctca cgctacctca
      421 ggggaagcta ccgtcttcta ctacaagatg aaaggagatt attaccgtta cttggctgag
      481 tttaagaccg agcaagagag gaaggaagct gctgaacagt ctctcaaagg ctatgaggct
      541 gcaacacaag ctgcaagcac tgagcttcct tcgacccatc caattcgtct tggccttgct
      601 cttaacttct ctgttttcta ctatgagatc atgaactctc ctgaaagagc ttgccatttg
      661 gctaagcaag ccttcgatga ggcgattgca gagttagata ctctaagtga ggagtcgtac
      721 aaggacagca ctttgatcat gcagctcctg agagacaacc tcaccctctg gacttctgat
      781 cttcctgaag atggaggaga agacaacatc aagactgaag aatccaagca agaacaggcc
      841 aagcctgccg atgccactga gaactgactc gaggat
//
