LOCUS       sequence-62485-e         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Sehnke PC, Laughner B, Cardasis H, Powell D, Ferl RJ
  TITLE     Exposed loop domains of complexed 14-3-3 proteins contribute to 
            structural diversity and functional specificity.
  JOURNAL   Plant Physiol. 2006 Feb;140(2):647-60. Epub 2006 Jan 11.
  PUBMED    16407442
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2006-02"; volume: "140(2)"; pages: "647-60. Epub 
            2006 Jan 11"
COMMENT     Sequence Label: pET15b-At 14-3-3 lambda
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atggcggcga cattaggcag agaccagtat gtgtacatgg cgaagctcgc cgagcaggcg
      121 gagcgttacg aagagatggt tcaattcatg gaacagctcg ttacaggcgc tactccagcg
      181 gaagagctca ccgttgaaga gaggaatctc ctctctgttg cttacaaaaa cgtgatcgga
      241 tctctacgcg ccgcctggag gatcgtgtct tcgattgagc agaaggaaga gagtaggaag
      301 aacgacgagc acgtgtcgct tgtcaaggat tacagatcta aagttgagtc tgagctttct
      361 tctgtttgct ctggaatcct taagctcctt gactcgcatc tgatcccatc tgctggagcg
      421 agtgagtcta aggtctttta cttgaagatg aaaggtgatt atcatcggta catggctgag
      481 tttaagtctg gtgatgagag gaaaactgct gctgaagata ccatgctcgc ttacaaagca
      541 gctcaggata tcgcagctgc ggatatggca cctactcatc cgataaggct tggtctggcc
      601 ctgaatttct cagtgttcta ctatgagatt ctcaattctt cagacaaagc ttgtaacatg
      661 gccaaacagg cttttgagga ggccatagct gagcttgaca ctctgggaga ggaatcctac
      721 aaagacagca ctctcataat gcagttgctg agggacaatt taaccctttg gacctccgat
      781 atgcaggagc agatggacga ggcctgagga tctagatgaa gggggggagg gttgttacgc
      841 gatgtttctg ccaccaaatc gatctcaaaa tccccataac ctttgctcaa aaactgtgaa
      901 aaaagattga agtgtttatg atgattatga ttgtgcacag cttgagattt atctactcta
      961 ctaaacctct gtgcgg
//
