LOCUS       sequence-62488-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human GSK3 beta constitutively active mutant
            with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Stambolic V, Woodgett JR
  TITLE     Mitogen inactivation of glycogen synthase kinase-3 beta in intact 
            cells via serine 9 phosphorylation.
  JOURNAL   Biochem J. 1994 Nov 1;303 ( Pt 3)(Pt 3):701-4. doi: 
            10.1042/bj3030701.
  PUBMED    7980435
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            J."; date: "1994-11-1"; volume: "303 ( Pt 3)(Pt 3):701-4. doi"; 
            pages: " 10.1042/bj3030701"
COMMENT     Sequence Label: HA GSK3 beta S9A pcDNA3
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
       61 gactcactat agggagaccc aagcttggta ccgagctcgg atccactagt aacggccgcc
      121 agtgtgctgg aattctgcag atcatgggaa ttcccctcga catggcaggg cggcccagaa
      181 ccaccgcctt tgcggagagc tgcaagccgg tgcagcagcc ttcagctttt ggcagcatga
      241 aagttagcag agacaaggac ggcagcaagg tgacaacagt ggtggcaact cctgggcagg
      301 gtccagacag gccacaagaa gtcagctata cagacactaa agtgattgga aatggatcat
      361 ttggtgtggt atatcaagcc aaactttgtg attcaggaga actggtcgcc atcaagaaag
      421 tattgcagga caagagattt aagaatcgag agctccagat catgagaaag ctagatcact
      481 gtaacatagt ccgattgcgt tatttcttct actccagtgg tgagaagaaa gatgaggtct
      541 atcttaatct ggtgctggac tatgttccgg aaacagtata cagagttgcc agacactata
      601 gtcgagccaa acagacgctc cctgtgattt atgtcaagtt gtatatgtat cagctgttcc
      661 gaagtttagc ctatatccat tcctttggaa tctgccatcg ggatattaaa ccgcagaacc
      721 tcttgttgga tcctgatact gctgtattaa aactctgtga ctttggaagt gcaaagcagc
      781 tggtccgagg agaacccaat gtttcgtata tctgttctcg gtactatagg gcaccagagt
      841 tgatctttgg agccactgat tatacctcta gtatagatgt atgg
//
