LOCUS       sequence-62490-e        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Kumar MS, Lu J, Mercer KL, Golub TR, Jacks T
  TITLE     Impaired microRNA processing enhances cellular transformation and 
            tumorigenesis.
  JOURNAL   Nat Genet. 2007 May;39(5):673-7. doi: 10.1038/ng2003.
  PUBMED    17401365
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Genet"; date: "2007-05"; volume: "39"; issue: "5"; pages: 
            "673-7"
COMMENT     Sequence Label: pSicoR human Dicer1
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..50
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     complement(255..275)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     protein_bind    279..312
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    565..682
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(980..1024)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aggccacttg tgtagcgcca agtgcccagc ggggctgcta aagcgcatgc tccagactgc
       61 cttgggaaaa gcgcctcccc tacccggtag aatttcgagg tcgacgaatt cggccgccta
      121 atggatccta gtactcgaga aaaaagcagc tctggatcat aatatctctt gaatattatg
      181 atccagagct gcaaacaagg cttttctcca agggatattt atagtctcaa aacacacaat
      241 tactttacag ttagggtgag tttccttttg tgctgtttat aacttcgtat aatgtatgct
      301 atacgaagtt atagaaatcc aagcctatca tgtaaaatgt agctagtatt aaaaagaaca
      361 gattatctgt cttttatcgc acattaagcc tctatagtta ctaggaaata ttatatgcaa
      421 attaaccggg gcaggggagt agccgagctt ctcccacaag tctgtgcgag ggggccggcg
      481 cgggcctaga gatggcgcgt cggatctcta gagcgggccc ggtactaacc aaactggatc
      541 tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt tttgtaattc
      601 tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac tgtacccccc
      661 aatcccccct tttcttttaa aattgtggat gaatactgcc atttgtctgc agaattggcg
      721 cacgcagtgc cgatccgttc actaatcgaa tggatctgtc tctgtctctc tctccacctt
      781 cttcttctat tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg
      841 ataatggtga atatccctgc ctaactctat tcactataga aagtacagca aaaactattc
      901 ttaaacctac caagcctcct actatcatta tgaataattt tatataccac agccaatttg
      961 ttatgttaaa ccaattccac aaacttgccc atttatctaa ttccaataat tcttgttcat
     1021 tcttttcttg ctggttttgc gattcttcaa ttaagga
//
