LOCUS       sequence-62491-e         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human GSK3 beta constitutively active mutant
            with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Stambolic V, Woodgett JR
  TITLE     Mitogen inactivation of glycogen synthase kinase-3 beta in intact 
            cells via serine 9 phosphorylation.
  JOURNAL   Biochem J. 1994 Nov 1;303 ( Pt 3)(Pt 3):701-4. doi: 
            10.1042/bj3030701.
  PUBMED    7980435
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            J."; date: "1994-11-1"; volume: "303 ( Pt 3)(Pt 3):701-4. doi"; 
            pages: " 10.1042/bj3030701"
COMMENT     Sequence Label: HA GSK3 beta S9A pcDNA3
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(203..229)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(209..235)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(670..681)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 ctatagaata gggccctcta gatgcatgct cgagcggccg ccagtgtgat ggatgactta
       61 attaattagg cctctcgagc tggagggatc cactagtgag ctccccaatt cggttttttt
      121 tttttttttt tttttttttt tttgggagct cgcccgggga tcctctagag gatccgtcgg
      181 gactgtacta gttgggacca cccaagcttg cgtaatctgg aacatcgtat gggtaactag
      241 tggagttgga agctgatgca gaagcagcat tattggtctg tccacggtct ccagtattag
      301 catctgacgc tgctgtggca tttgtggggg ttgaagcagc tgcttgaatc cgagcatgag
      361 gaggaataag gatggtagcc agaggtggat tacttgacag ttcttgagtg gtgaagttga
      421 agagtgcagg tgtgtctcgc ccatttggta gtttgacatt tgggtcccgt aattcatcaa
      481 aaaatgaatg tgcacaagct tccagtggtg ttagtcgggc agttggtgta tactccagca
      541 gacggctaca cagtgcaatt gcctccggtg gagttcgggg tcggaagacc ttagtccaag
      601 gatgtgcctt aatttgaggg aatttaaatt ctgtgtagtt tgggttcatt tctctgattt
      661 gctcccttgt tggagttccc aggaccttga ttatttctac caactgatcc acaccactat
      721 cccctggaaa ta
//
