LOCUS       sequence-62492-e        5675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of CaMKIV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Sun P, Enslen H, Myung PS, Maurer RA
  TITLE     Differential activation of CREB by Ca2+/calmodulin-dependent protein
            kinases type II and type IV involves phosphorylation of a site that 
            negatively regulates activity.
  JOURNAL   Genes Dev. 1994 Nov 1;8(21):2527-39.
  PUBMED    7958915
REFERENCE   2  (bases 1 to 5675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "1994-11-1"; volume: "8(21)"; pages: "2527-39"
COMMENT     Sequence Label: pRSV-CaMKIV
FEATURES             Location/Qualifiers
     source          1..5675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          1510..1575
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1705..1725
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2150..2284
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2187..2206)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2241..2260
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3051..3069)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3137..3241
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3242..3310,3311..4102)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVRYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3460..3479)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4273..4861
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4762..4781
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5015..5032
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5113..5132)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5409..5671
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 agcttaccat gctcaaagtc acggtgccct cctgtccctc ctcgccctgc tcctcggtca
       61 ccgccagtac tgagaacctc gtcccggatt actggatcga cggctctaac cgagatcctc
      121 tgggcgattt cttcgaggtg gagtcagagc tgggacgggg tgctacatcc attgtgtaca
      181 gatgcaaaca gaaggggacc cagaagccct atgctctcaa agtgttaaag aaaacagtgg
      241 acaagaagat tgtgagaaca gaaataggag ttctcctgcg tctctcacac ccgaacatca
      301 taaaactcaa ggaaatattc gaaaccccca cagaaatcag cctggtcctt gagctggtca
      361 caggaggaga actgtttgac aggattgtgg agaagggata ctacagtgag cgcgatgcgc
      421 gtgacgcggt gaagcagatc ctggaggccg ttgcttacct gcatgaaaat gggattgtcc
      481 atcgtgacct caaaccagag aatcttcttt atgcaactcc agcccctgat gcacccctca
      541 aaattgctga ttttggactt tcaaaaattg tggaacatca agtgctcatg aagacagtgt
      601 gtggaacccc ggggtactgc gcacctgaga ttctccgagg ctgtgcctac ggacctgagg
      661 tggacatgtg gtctgtagga ataatcacct acatcctact ttgtggattt gaaccattct
      721 atgacgagcg aggtgatcag ttcatgttca ggagaattct gaattgtgaa tattacttta
      781 tctccccctg gtgggatgaa gtgtctttaa atgccaagga cttggtcaaa aagctcattg
      841 ttttggatcc caagaaacgg ctgactacat ttcaagccct ccaacaccca tgggtcacag
      901 gtaaagcggc caactttgtt cacatggaca ctgctcagaa gaaacttcaa gaattcaatg
      961 ctcggcgcaa gcttaaggca gcggtgaagg ctgtggtggc ctcttctcgg ctgggaagtg
     1021 ccagcagtag ccacaccagc atccaagaga accacaaggc cagctcggat ccaccttcaa
     1081 cccaagatgc caaggacagc acagatcttc tgggaaagaa aatgcaagag gaggaccaag
     1141 aggaggacca agtggaggcc gaggcttcag ccgatgagat gaggaagctg cagtccgagg
     1201 aggtggagaa agatgcaggt gtaaaagagg aggagacctc cagtatggtg cctcaggatc
     1261 cagaggatga gctggaaaca gatgacccag agatgaagag ggattcagag gagaagctga
     1321 agagtgtgga ggaagaaatg gaccccatga ctgaggagga agcccctgac gcgggacttg
     1381 gggttccaca gcaggatgcg attcagccag agtactaatc tagagcggcc gcagatcttt
     1441 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     1501 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     1561 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
     1621 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
     1681 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
     1741 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
     1801 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
     1861 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt
     1921 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
     1981 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
     2041 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     2101 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     2161 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     2221 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     2281 gatccccagg aagctcctct gtgtcctcat aaaccctaac ctcctctact tgagaggaca
     2341 ttccaatcat aggctgccca tccaccctct gtgtcctcct gttaattagg tcacttaaca
     2401 aaaaggaaat tgggtagggg tttttcacag accgctttct aagggtaatt ttaaaatatc
     2461 tgggaagtcc cttccactgc tgtgttccag aagtgttggt aaacagccca caaatgtcaa
     2521 cagcagaaac atacaagctg tcagctttgc acaagggccc aacaccctgc tcatcaagaa
     2581 gcactgtggt tgctgtgtta gtaatgtgca aaacaggagg cacattttcc ccacctgtgt
     2641 aggttccaaa atatctagtg ttttcatttt tacttggatc aggaacccag cactccactg
     2701 gataagcatt atccttatcc aaaacagcct tgtggtcagt gttcatctgc tgactgtcaa
     2761 ctgtagcatt ttttggggtt acagtttgag caggatattt ggtcctgtag tttgctaaca
     2821 caccctgcag ctccaaaggt tccccaccaa cagcaaaaaa atgaaaattt gacccttgaa
     2881 tgggttttcc agcaccattt tcatgagttt tttgtgtccc tgaatgcaag tttaacatag
     2941 cagttacccc aataacctca gttttaacag taacagcttc ccacatcaaa atatttccac
     3001 aggttaagtc ctcatttaaa ttaggcaaag gaattcttga agacgaaagg gcctcgtgat
     3061 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     3121 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     3181 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     3241 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     3301 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     3361 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     3421 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     3481 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     3541 ggttagatac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     3601 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     3661 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     3721 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     3781 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     3841 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     3901 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     3961 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     4021 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     4081 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     4141 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     4201 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     4261 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4321 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4381 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     4441 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4501 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4561 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4621 ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac
     4681 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4741 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4801 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4861 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4921 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     4981 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     5041 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     5101 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     5161 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     5221 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
     5281 tgtacgggcc agatatacgc gtatctgagg ggactagggt gtgtttaggc gaaaagcggg
     5341 gcttcggttg tacgcggtta ggagtcccct caggatatag tagtttcgct tttgcatagg
     5401 gagggggaaa tgtagtctta tgcaatacac ttgtagtctt gcaacatggt aacgatgagt
     5461 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
     5521 tggtacgatc gtgccttatt aggaaggcaa cagacaggtc tgacatggat tggacgaacc
     5581 actgaattcc gcattgcaga gataattgta tttaagtgcc tagctcgata caataaacgc
     5641 catttgacca ttcaccacat tggtgtgcac ctcca
//
