LOCUS       sequence-62493-e        5193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of constit active CaMKIV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Sun P, Enslen H, Myung PS, Maurer RA
  TITLE     Differential activation of CREB by Ca2+/calmodulin-dependent protein
            kinases type II and type IV involves phosphorylation of a site that 
            negatively regulates activity.
  JOURNAL   Genes Dev. 1994 Nov 1;8(21):2527-39.
  PUBMED    7958915
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "1994-11-1"; volume: "8(21)"; pages: "2527-39"
COMMENT     Sequence Label: pRSV-CaMKIV(1-313)
FEATURES             Location/Qualifiers
     source          1..5193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          1028..1093
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1223..1243
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1668..1802
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1705..1724)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1759..1778
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2569..2587)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2655..2759
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2760..2828,2829..3620)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVRYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2978..2997)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3791..4379
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4280..4299
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4533..4550
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4631..4650)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4927..5189
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 agcttaccat gctcaaagtc acggtgccct cctgtccctc ctcgccctgc tcctcggtca
       61 ccgccagtac tgagaacctc gtcccggatt actggatcga cggctctaac cgagatcctc
      121 tgggcgattt cttcgaggtg gagtcagagc tgggacgggg tgctacatcc attgtgtaca
      181 gatgcaaaca gaaggggacc cagaagccct atgctctcaa agtgttaaag aaaacagtgg
      241 acaagaagat tgtgagaaca gaaataggag ttctcctgcg tctctcacac ccgaacatca
      301 taaaactcaa ggaaatattc gaaaccccca cagaaatcag cctggtcctt gagctggtca
      361 caggaggaga actgtttgac aggattgtgg agaagggata ctacagtgag cgcgatgcgc
      421 gtgacgcggt gaagcagatc ctggaggccg ttgcttacct gcatgaaaat gggattgtcc
      481 atcgtgacct caaaccagag aatcttcttt atgcaactcc agcccctgat gcacccctca
      541 aaattgctga ttttggactt tcaaaaattg tggaacatca agtgctcatg aagacagtgt
      601 gtggaacccc ggggtactgc gcacctgaga ttctccgagg ctgtgcctac ggacctgagg
      661 tggacatgtg gtctgtagga ataatcacct acatcctact ttgtggattt gaaccattct
      721 atgacgagcg aggtgatcag ttcatgttca ggagaattct gaattgtgaa tattacttta
      781 tctccccctg gtgggatgaa gtgtctttaa atgccaagga cttggtcaaa aagctcattg
      841 ttttggatcc caagaaacgg ctgactacat ttcaagccct ccaacaccca tgggtcacag
      901 gtaaagcggc caactttgtt cacatggaca ctgctcagaa gaaactttaa agatctttgt
      961 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag
     1021 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt
     1081 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt
     1141 taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc
     1201 tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt
     1261 tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg
     1321 ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa
     1381 atattctgta acctttataa gtaggcataa cagttataat cataacatac tgttttttct
     1441 tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt
     1501 tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag
     1561 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     1621 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     1681 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     1741 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     1801 tccccaggaa gctcctctgt gtcctcataa accctaacct cctctacttg agaggacatt
     1861 ccaatcatag gctgcccatc caccctctgt gtcctcctgt taattaggtc acttaacaaa
     1921 aaggaaattg ggtaggggtt tttcacagac cgctttctaa gggtaatttt aaaatatctg
     1981 ggaagtccct tccactgctg tgttccagaa gtgttggtaa acagcccaca aatgtcaaca
     2041 gcagaaacat acaagctgtc agctttgcac aagggcccaa caccctgctc atcaagaagc
     2101 actgtggttg ctgtgttagt aatgtgcaaa acaggaggca cattttcccc acctgtgtag
     2161 gttccaaaat atctagtgtt ttcattttta cttggatcag gaacccagca ctccactgga
     2221 taagcattat ccttatccaa aacagccttg tggtcagtgt tcatctgctg actgtcaact
     2281 gtagcatttt ttggggttac agtttgagca ggatatttgg tcctgtagtt tgctaacaca
     2341 ccctgcagct ccaaaggttc cccaccaaca gcaaaaaaat gaaaatttga cccttgaatg
     2401 ggttttccag caccattttc atgagttttt tgtgtccctg aatgcaagtt taacatagca
     2461 gttaccccaa taacctcagt tttaacagta acagcttccc acatcaaaat atttccacag
     2521 gttaagtcct catttaaatt aggcaaagga attcttgaag acgaaagggc ctcgtgatac
     2581 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     2641 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     2701 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     2761 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     2821 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     2881 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     2941 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     3001 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     3061 ttagatactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     3121 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     3181 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     3241 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     3301 ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     3361 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     3421 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     3481 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     3541 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     3601 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     3661 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     3721 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3781 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3841 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3901 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     3961 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4021 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4081 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4141 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc
     4201 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4261 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4321 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4381 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4441 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     4501 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     4561 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     4621 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     4681 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     4741 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     4801 tacgggccag atatacgcgt atctgagggg actagggtgt gtttaggcga aaagcggggc
     4861 ttcggttgta cgcggttagg agtcccctca ggatatagta gtttcgcttt tgcataggga
     4921 gggggaaatg tagtcttatg caatacactt gtagtcttgc aacatggtaa cgatgagtta
     4981 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     5041 gtacgatcgt gccttattag gaaggcaaca gacaggtctg acatggattg gacgaaccac
     5101 tgaattccgc attgcagaga taattgtatt taagtgccta gctcgataca ataaacgcca
     5161 tttgaccatt caccacattg gtgtgcacct cca
//
