LOCUS       sequence-62496-e        5729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of CaMKII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Sun P, Enslen H, Myung PS, Maurer RA
  TITLE     Differential activation of CREB by Ca2+/calmodulin-dependent protein
            kinases type II and type IV involves phosphorylation of a site that 
            negatively regulates activity.
  JOURNAL   Genes Dev. 1994 Nov 1;8(21):2527-39.
  PUBMED    7958915
REFERENCE   2  (bases 1 to 5729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "1994-11-1"; volume: "8(21)"; pages: "2527-39"
COMMENT     Sequence Label: pRSV-CaMKII
FEATURES             Location/Qualifiers
     source          1..5729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          1565..1630
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1760..1780
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2205..2339
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2242..2261)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2296..2315
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3106..3124)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3192..3296
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3297..3365,3366..4157)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVRYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3515..3534)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4328..4916
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4817..4836
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5070..5087
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5168..5187)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5464..5726
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 aagcttgcca ccatggctac catcacctgc acccgattca cggaagagta ccagctcttc
       61 gaggaactgg gaaagggagc cttctccgtg gtgcgcaggt gtgtgaaggt gctggctggc
      121 caggagtatg ctgccaagat tatcaacacc aagaagctct cagccagaga tcaccagaag
      181 ttggaacgcg aggcccgcat ctgccgcttg ttgaagcacc ccaatatcgt ccgactccat
      241 gacagcatct ccgaggaggg gcaccactac cttatcttcg atctggtcac tggtggggag
      301 ctgttcgaag acattgtggc ccgggagtat tacagtgagg ctgatgccag ccactgtatc
      361 cagcagatcc tggaggctgt gctacactgt caccagatgg gggtggtgca tcgcgacctg
      421 aagcctgaga atctgttgct ggcttcgaag ctcaagggtg ctgcggtgaa gctggcagac
      481 tttggcctgg ccatagaggt tgagggagag cagcaggcat ggtttgggtt cgcagggaca
      541 cctggatacc tctccccaga agtgctgcgg aaggacccat acgggaagcc tgtggacctg
      601 tgggcctgtg gcgtcatcct gtatatcttg ctggttgggt atcccccatt ctgggatgag
      661 gaccagcacc gcctgtacca gcagatcaaa gctggtgcct acgatttccc atcaccagaa
      721 tgggacaccg tcaccccgga agccaaggat ctgatcaata agatgctgac catcaacccg
      781 tccaaacgca tcacggccgc tgaggctctc aagcacccct ggatctcgca ccgctccact
      841 gtggcctcct gcatgcacag acaggagacc gtggactgcc tgaagaagtt caatgccagg
      901 aggaaactga agggagccat cctcaccact atgctggcca ccaggaactt ctccggaggg
      961 aagagtggag gaaacaagaa gaatgatggc gtgaaggaat cctctgagag caccaacacc
     1021 accatcgagg atgaagacac caaagtgcgc aaacaggaaa ttatcaaagt gacagagcag
     1081 ctgatcgaag ccataagcaa tggagacttt gaatcctaca cgaagatgtg cgaccctgga
     1141 atgacagcct ttgaaccgga ggccctgggg aacctggtcg agggcctgga ctttcatcga
     1201 ttctattttg aaaacctgtg gtcccggaac agcaagcccg tgcacaccac catcctgaac
     1261 cctcacatcc acctgatggg tgacgagtca gcctgcatcg cctacatccg catcactcag
     1321 tacctggatg cgggtggcat cccccgcacg gcccagtcag aggagacccg tgtctggcac
     1381 cgcagggatg gaaaatggca gatcgtccac ttccacagat ctggggcgcc ctccgtcctg
     1441 ccccattgaa ggaccaggcc agggtccctg cgctctagag cggccgcaga tctttgtgaa
     1501 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
     1561 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
     1621 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
     1681 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
     1741 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
     1801 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
     1861 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
     1921 ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt tttttcttac
     1981 tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag
     2041 ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga
     2101 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     2161 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     2221 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     2281 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc
     2341 ccaggaagct cctctgtgtc ctcataaacc ctaacctcct ctacttgaga ggacattcca
     2401 atcataggct gcccatccac cctctgtgtc ctcctgttaa ttaggtcact taacaaaaag
     2461 gaaattgggt aggggttttt cacagaccgc tttctaaggg taattttaaa atatctggga
     2521 agtcccttcc actgctgtgt tccagaagtg ttggtaaaca gcccacaaat gtcaacagca
     2581 gaaacataca agctgtcagc tttgcacaag ggcccaacac cctgctcatc aagaagcact
     2641 gtggttgctg tgttagtaat gtgcaaaaca ggaggcacat tttccccacc tgtgtaggtt
     2701 ccaaaatatc tagtgttttc atttttactt ggatcaggaa cccagcactc cactggataa
     2761 gcattatcct tatccaaaac agccttgtgg tcagtgttca tctgctgact gtcaactgta
     2821 gcattttttg gggttacagt ttgagcagga tatttggtcc tgtagtttgc taacacaccc
     2881 tgcagctcca aaggttcccc accaacagca aaaaaatgaa aatttgaccc ttgaatgggt
     2941 tttccagcac cattttcatg agttttttgt gtccctgaat gcaagtttaa catagcagtt
     3001 accccaataa cctcagtttt aacagtaaca gcttcccaca tcaaaatatt tccacaggtt
     3061 aagtcctcat ttaaattagg caaaggaatt cttgaagacg aaagggcctc gtgatacgcc
     3121 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     3181 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     3241 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     3301 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     3361 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     3421 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     3481 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
     3541 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggtta
     3601 gatactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     3661 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     3721 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     3781 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     3841 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     3901 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     3961 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     4021 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     4081 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     4141 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     4201 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     4261 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4321 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4381 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4441 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     4501 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4561 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4621 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4681 gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc
     4741 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4801 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4861 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4921 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4981 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     5041 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     5101 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     5161 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     5221 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     5281 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
     5341 gggccagata tacgcgtatc tgaggggact agggtgtgtt taggcgaaaa gcggggcttc
     5401 ggttgtacgc ggttaggagt cccctcagga tatagtagtt tcgcttttgc atagggaggg
     5461 ggaaatgtag tcttatgcaa tacacttgta gtcttgcaac atggtaacga tgagttagca
     5521 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     5581 cgatcgtgcc ttattaggaa ggcaacagac aggtctgaca tggattggac gaaccactga
     5641 attccgcatt gcagagataa ttgtatttaa gtgcctagct cgatacaata aacgccattt
     5701 gaccattcac cacattggtg tgcacctcc
//
