LOCUS       sequence-62499-e        5129 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of constit active CaMKII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Sun P, Enslen H, Myung PS, Maurer RA
  TITLE     Differential activation of CREB by Ca2+/calmodulin-dependent protein
            kinases type II and type IV involves phosphorylation of a site that 
            negatively regulates activity.
  JOURNAL   Genes Dev. 1994 Nov 1;8(21):2527-39.
  PUBMED    7958915
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "1994-11-1"; volume: "8(21)"; pages: "2527-39"
COMMENT     Sequence Label: pRSV-CaMKII(1-290)
FEATURES             Location/Qualifiers
     source          1..5129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      2..11
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          960..1025
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1155..1175
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1600..1734
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1637..1656)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1691..1710
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2501..2519)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2587..2691
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2692..2760,2761..3552)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVRYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2910..2929)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3723..4311
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4212..4231
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4465..4482
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4563..4582)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4859..5121
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 tgccaccatg gctaccatca cctgcacccg attcacggaa gagtaccagc tcttcgagga
       61 actgggaaag ggagccttct ccgtggtgcg caggtgtgtg aaggtgctgg ctggccagga
      121 gtatgctgcc aagattatca acaccaagaa gctctcagcc agagatcacc agaagttgga
      181 acgcgaggcc cgcatctgcc gcttgttgaa gcaccccaat atcgtccgac tccatgacag
      241 catctccgag gaggggcacc actaccttat cttcgatctg gtcactggtg gggagctgtt
      301 cgaagacatt gtggcccggg agtattacag tgaggctgat gccagccact gtatccagca
      361 gatcctggag gctgtgctac actgtcacca gatgggggtg gtgcatcgcg acctgaagcc
      421 tgagaatctg ttgctggctt cgaagctcaa gggtgctgcg gtgaagctgg cagactttgg
      481 cctggccata gaggttgagg gagagcagca ggcatggttt gggttcgcag ggacacctgg
      541 atacctctcc ccagaagtgc tgcggaagga cccatacggg aagcctgtgg acctgtgggc
      601 ctgtggcgtc atcctgtata tcttgctggt tgggtatccc ccattctggg atgaggacca
      661 gcaccgcctg taccagcaga tcaaagctgg tgcctacgat ttcccatcac cagaatggga
      721 caccgtcacc ccggaagcca aggatctgat caataagatg ctgaccatca acccgtccaa
      781 acgcatcacg gccgctgagg ctctcaagca cccctggatc tcgcaccgct ccactgtggc
      841 ctcctgcatg cacagacagg agaccgtgga ctgcctgtag taggatcttt gtgaaggaac
      901 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
      961 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
     1021 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
     1081 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
     1141 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
     1201 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
     1261 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg
     1321 taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac
     1381 acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt
     1441 taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata
     1501 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     1561 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     1621 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     1681 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccccagg
     1741 aagctcctct gtgtcctcat aaaccctaac ctcctctact tgagaggaca ttccaatcat
     1801 aggctgccca tccaccctct gtgtcctcct gttaattagg tcacttaaca aaaaggaaat
     1861 tgggtagggg tttttcacag accgctttct aagggtaatt ttaaaatatc tgggaagtcc
     1921 cttccactgc tgtgttccag aagtgttggt aaacagccca caaatgtcaa cagcagaaac
     1981 atacaagctg tcagctttgc acaagggccc aacaccctgc tcatcaagaa gcactgtggt
     2041 tgctgtgtta gtaatgtgca aaacaggagg cacattttcc ccacctgtgt aggttccaaa
     2101 atatctagtg ttttcatttt tacttggatc aggaacccag cactccactg gataagcatt
     2161 atccttatcc aaaacagcct tgtggtcagt gttcatctgc tgactgtcaa ctgtagcatt
     2221 ttttggggtt acagtttgag caggatattt ggtcctgtag tttgctaaca caccctgcag
     2281 ctccaaaggt tccccaccaa cagcaaaaaa atgaaaattt gacccttgaa tgggttttcc
     2341 agcaccattt tcatgagttt tttgtgtccc tgaatgcaag tttaacatag cagttacccc
     2401 aataacctca gttttaacag taacagcttc ccacatcaaa atatttccac aggttaagtc
     2461 ctcatttaaa ttaggcaaag gaattcttga agacgaaagg gcctcgtgat acgcctattt
     2521 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     2581 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     2641 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     2701 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     2761 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     2821 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     2881 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     2941 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttagatac
     3001 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     3061 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     3121 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     3181 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     3241 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     3301 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     3361 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     3421 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     3481 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     3541 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     3601 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     3661 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3721 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3781 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3841 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     3901 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3961 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     4021 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     4081 acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa
     4141 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     4201 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     4261 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     4321 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     4381 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     4441 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     4501 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     4561 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
     4621 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
     4681 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
     4741 agatatacgc gtatctgagg ggactagggt gtgtttaggc gaaaagcggg gcttcggttg
     4801 tacgcggtta ggagtcccct caggatatag tagtttcgct tttgcatagg gagggggaaa
     4861 tgtagtctta tgcaatacac ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     4921 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     4981 gtgccttatt aggaaggcaa cagacaggtc tgacatggat tggacgaacc actgaattcc
     5041 gcattgcaga gataattgta tttaagtgcc tagctcgata caataaacgc catttgacca
     5101 ttcaccacat tggtgtgcac ctccaagct
//
