LOCUS       sequence-62500-e        4523 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of PKI (PKA inhibitor).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4523)
  TITLE     RSV-PKI
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRSV-PKI-v2
FEATURES             Location/Qualifiers
     source          1..4523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(257..273)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(257..273)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     intron          359..424
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             554..574
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    999..1133
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1036..1055)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1090..1109
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1900..1918)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1986..2090
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2091..2159,2160..2951)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVRYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2309..2328)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3122..3710
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3611..3630
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3864..3881
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3962..3981)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4258..4520
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 aagcttgcca ccatgggaac tgatgtcgaa actacttatg ccgattttat cgcctcagga
       61 cgtactggtc gacgtaacgc catccacgat atcctggtct cgagtgcttc cggaaactca
      121 aacgaactgg ccctgaagct ggctggtcta gatattaaca aaacagaagg agaggaagat
      181 gctcaaagat cttcaactga acaatccgga gaagctcaag gagaagctgc taagtccgag
      241 tcatagtagg gatctggatc cactagttct agagcggccg cagatctttg tgaaggaacc
      301 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
      361 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
      421 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
      481 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
      541 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
      601 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
      661 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt
      721 aacctttata agtaggcata acagttataa tcataacata ctgttttttc ttactccaca
      781 caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt
      841 aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa
      901 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
      961 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     1021 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     1081 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccccagga
     1141 agctcctctg tgtcctcata aaccctaacc tcctctactt gagaggacat tccaatcata
     1201 ggctgcccat ccaccctctg tgtcctcctg ttaattaggt cacttaacaa aaaggaaatt
     1261 gggtaggggt ttttcacaga ccgctttcta agggtaattt taaaatatct gggaagtccc
     1321 ttccactgct gtgttccaga agtgttggta aacagcccac aaatgtcaac agcagaaaca
     1381 tacaagctgt cagctttgca caagggccca acaccctgct catcaagaag cactgtggtt
     1441 gctgtgttag taatgtgcaa aacaggaggc acattttccc cacctgtgta ggttccaaaa
     1501 tatctagtgt tttcattttt acttggatca ggaacccagc actccactgg ataagcatta
     1561 tccttatcca aaacagcctt gtggtcagtg ttcatctgct gactgtcaac tgtagcattt
     1621 tttggggtta cagtttgagc aggatatttg gtcctgtagt ttgctaacac accctgcagc
     1681 tccaaaggtt ccccaccaac agcaaaaaaa tgaaaatttg acccttgaat gggttttcca
     1741 gcaccatttt catgagtttt ttgtgtccct gaatgcaagt ttaacatagc agttacccca
     1801 ataacctcag ttttaacagt aacagcttcc cacatcaaaa tatttccaca ggttaagtcc
     1861 tcatttaaat taggcaaagg aattcttgaa gacgaaaggg cctcgtgata cgcctatttt
     1921 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     1981 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     2041 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     2101 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     2161 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     2221 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     2281 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     2341 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttagatact
     2401 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     2461 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     2521 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     2581 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa
     2641 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     2701 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     2761 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     2821 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     2881 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     2941 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     3001 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     3061 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3121 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3181 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3241 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     3301 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3361 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3421 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3481 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag
     3541 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3601 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3661 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3721 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3781 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3841 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     3901 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     3961 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     4021 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     4081 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
     4141 gatatacgcg tatctgaggg gactagggtg tgtttaggcg aaaagcgggg cttcggttgt
     4201 acgcggttag gagtcccctc aggatatagt agtttcgctt ttgcataggg agggggaaat
     4261 gtagtcttat gcaatacact tgtagtcttg caacatggta acgatgagtt agcaacatgc
     4321 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     4381 tgccttatta ggaaggcaac agacaggtct gacatggatt ggacgaacca ctgaattccg
     4441 cattgcagag ataattgtat ttaagtgcct agctcgatac aataaacgcc atttgaccat
     4501 tcaccacatt ggtgtgcacc tcc
//
