LOCUS       sequence-62501-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Pertz O, Hodgson L, Klemke RL, Hahn KM
  TITLE     Spatiotemporal dynamics of RhoA activity in migrating cells.
  JOURNAL   Nature. 2006 Apr 20. 440(7087):1069-72.
  PUBMED    16547516
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2006 Apr 20. 440(7087):1069-72."
COMMENT     Sequence Label: pTriEx-RhoA FLARE.sc Biosensor T19N
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        21..130
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             151..168
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(433..435,436..438,439..897)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITA"
     primer_bind     complement(478..499)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(739..758)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 attccccgga gttaatccgg gacctttaat tcaacccaac acaatatatt atagttaaat
       61 aagaattatt atcaaatcat ttgtatatta attaaaatac tatactgtaa attacatttt
      121 atttacaatc aaaggagata taccatggca caccatcacc accatcacgg tagtggcatc
      181 ctggaggacc tcaatatgct ctacatccgg cagatggcac tcagcctgga ggacacagag
      241 ctgcagagga aactagatca tgagatccgg atgagggatg gggcctgcaa gctgctggca
      301 gcctgctccc agcgagagca ggctctggaa gccaccaaga gcctgctggt gtgcaacagc
      361 cgtattctca gctacatggg tgagctgcag cggcgaaagg aggcccaggt gctggagaag
      421 acaggcgggg gaatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      481 gtcgagctgg acggcgacgt aaacggccac aggttcagcg tgtccggcga gggcgagggc
      541 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      601 ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc
      661 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      721 cgtaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      781 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      841 atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac
      901 aagcagaaga acggcatcaa ggcccacttc aagatccgcc aca
//
