LOCUS       sequence-62502-e        4517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of intactive PKI (PKA inhibitor) mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4517)
  TITLE     RSV-PKI
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRSV-PKImut-v2
FEATURES             Location/Qualifiers
     source          1..4517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(251..267)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(251..267)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     intron          353..418
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             548..568
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    993..1127
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1030..1049)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1084..1103
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1894..1912)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1980..2084
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2085..2153,2154..2945)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVRYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2303..2322)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3116..3704
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3605..3624
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3858..3875
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3956..3975)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4252..4514
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 aagcttgcca ccatgggaac tgatgtcgaa actacttatg ccgattttat cgcctcagga
       61 cgtactggtg gaggtaacgc catccacgat atcctggtct cgagtgcttc cggaaactca
      121 aacgaactgg ccctgaagct ggctggtcta gatattaaca aaacagaagg agaggaagat
      181 gctcaaagat cttcaactga acaatccgga gaagctcaag gagaagctgc taagtccgag
      241 tcatagtagg gatccactag ttctagagcg gccgcagatc tttgtgaagg aaccttactt
      301 ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
      361 aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
      421 tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct
      481 gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc
      541 tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct
      601 aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac
      661 cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt
      721 tataagtagg cataacagtt ataatcataa catactgttt tttcttactc cacacaggca
      781 tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg
      841 taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc
      901 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
      961 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     1021 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     1081 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcccc aggaagctcc
     1141 tctgtgtcct cataaaccct aacctcctct acttgagagg acattccaat cataggctgc
     1201 ccatccaccc tctgtgtcct cctgttaatt aggtcactta acaaaaagga aattgggtag
     1261 gggtttttca cagaccgctt tctaagggta attttaaaat atctgggaag tcccttccac
     1321 tgctgtgttc cagaagtgtt ggtaaacagc ccacaaatgt caacagcaga aacatacaag
     1381 ctgtcagctt tgcacaaggg cccaacaccc tgctcatcaa gaagcactgt ggttgctgtg
     1441 ttagtaatgt gcaaaacagg aggcacattt tccccacctg tgtaggttcc aaaatatcta
     1501 gtgttttcat ttttacttgg atcaggaacc cagcactcca ctggataagc attatcctta
     1561 tccaaaacag ccttgtggtc agtgttcatc tgctgactgt caactgtagc attttttggg
     1621 gttacagttt gagcaggata tttggtcctg tagtttgcta acacaccctg cagctccaaa
     1681 ggttccccac caacagcaaa aaaatgaaaa tttgaccctt gaatgggttt tccagcacca
     1741 ttttcatgag ttttttgtgt ccctgaatgc aagtttaaca tagcagttac cccaataacc
     1801 tcagttttaa cagtaacagc ttcccacatc aaaatatttc cacaggttaa gtcctcattt
     1861 aaattaggca aaggaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     1921 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     1981 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     2041 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     2101 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     2161 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     2221 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     2281 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     2341 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttaga tactcaccag
     2401 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     2461 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     2521 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     2581 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
     2641 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     2701 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     2761 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     2821 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     2881 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     2941 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     3001 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     3061 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3121 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3181 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3241 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     3301 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3361 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3421 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3481 ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa
     3541 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3601 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3661 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3721 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3781 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3841 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     3901 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     3961 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     4021 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     4081 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
     4141 cgcgtatctg aggggactag ggtgtgttta ggcgaaaagc ggggcttcgg ttgtacgcgg
     4201 ttaggagtcc cctcaggata tagtagtttc gcttttgcat agggaggggg aaatgtagtc
     4261 ttatgcaata cacttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     4321 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     4381 attaggaagg caacagacag gtctgacatg gattggacga accactgaat tccgcattgc
     4441 agagataatt gtatttaagt gcctagctcg atacaataaa cgccatttga ccattcacca
     4501 cattggtgtg cacctcc
//
