LOCUS       sequence-62507-e        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  TITLE     Trono Lab Constitutive Lentiviral Plasmids
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pWPXLd
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    110..227
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     intron          284..487
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(307..327)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 gaacagctcc tcgcccttgc tcaccatggt ggcgaccggt agcgctagga cgcgtaagct
       61 taggcctgga tcccgtagtt taaacctcga ggctagtctc gtgatcgata aaattttgaa
      121 tttttgtaat ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta
      181 ttctttcccc tgcactgtac cccccaatcc ccccttttct tttaaaagtt aaccgatacc
      241 gtcgagatta attaaattta aatgtcgaaa ttcctcacga cacctgaaat ggaagaaaaa
      301 aactttgaac cactgtctga ggcttgagaa tgaaccaaga tccaaactca aaaagggcaa
      361 attccaagga gaattacatc aagtgccaag ctggcctaac ttcagtctcc acccactcag
      421 tgtggggaaa ctccatcgca taaaacccct ccccccaacc taaagacgac gtactccaaa
      481 agctcgagaa ctaatcgagg tgcctggacg gcgcccggta ctccgtggag tcacatgaag
      541 cgacggctga ggacggaaag gcccttttcc tttgtgtggg tgactcaccc gcccgctctc
      601 ccgagcgccg cgtcctccat tttgagctcc ctgcagcagg gccgggaagc ggccatcttt
      661 ccgctcacgc aactggtgcc gaccgggcca gccttgccgc ccagggcggg gcgatacacg
      721 gcggcgcgag gccaggcacc agagcaggcc ggccagcttg agactacccc cgtccgattc
      781 tcggtggccg cgctcgcagg ccccgcctcg ccgaacatgt gcgctgggac gcacgggccc
      841 cgtcgccgcc cgcggcccca aaaaccgaaa taccagtgtg cagatcttgg cccgcattta
      901 caagactatc ttgccagaaa aaaagcgtcg cagcaggtca tcaaaaattt taaatggcta
      961 gagacttatc gaaagcagcg agacaggcgc gaaggtgcca ccagattcgc acgcggcggc
     1021 cccagcgccc
//
