LOCUS       sequence-62509-e         646 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Mao CA, Cho JH, Wang J, Gao Z, Pan P, Tsai WW, Frishman LJ, Klein WH
  TITLE     Reprogramming amacrine and photoreceptor progenitors into retinal 
            ganglion cells by replacing Neurod1 with Atoh7.
  JOURNAL   Development. 2013 Feb;140(3):541-51. doi: 10.1242/dev.085886.
  PUBMED    23293286
REFERENCE   2  (bases 1 to 646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2013-02"; volume: "140"; issue: 
            "3"; pages: "541-51"
COMMENT     Sequence Label: Neurod1-Atoh7HA3
FEATURES             Location/Qualifiers
     source          1..646
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..71
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(72..105)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggaaaagcgc ctcccctacc cggtagaatt cggatccgaa gttcctattc tctagaaagt
       61 ataggaactt cataacttcg tatagcatac attatacgaa gttgtcgtga ctgagaaaac
      121 cctggcgacc ggtgcgtatt tatcgaattc ggcacgagga tttcgtcacc ccgaaaagca
      181 gtctcaggct ttcccagaga actggaaagg ctttctatcc ccgaccccca agaacgatcc
      241 gcagcaaact gcaagaaagt ttcctcctcg ttcaacttta cgtgtttggg gtggagtggg
      301 gaggaatgac tttttcctga gcatcacttc cactgcatct gggcaaggga agggacttcc
      361 cagcaggacc caagctaggg tcccacacag tgtcctctgg ccactgactg actgcacgtg
      421 agtcgctccg tctgtgtctt attcactctt gggagtcctg cagagcggca gcgtggatga
      481 agtcggcctg caaaccccac ggccctccgg cgggagctcg cggcgcgccc ccgtgcgcgg
      541 gcgcagccga gcgcgcggtc tcgtgcgcgg ggcccgggcg gctggagagc gcggcgcgca
      601 ggcgtctggc ggccaacgcg cgcgagcggc gccgcatgca ggggct
//
