LOCUS       sequence-62510-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Kamitani T, Kito K, Nguyen HP, Yeh ET
  TITLE     Characterization of NEDD8, a developmentally down-regulated 
            ubiquitin-like protein.
  JOURNAL   J Biol Chem. 1997 Nov 7. 272(45):28557-62.
  PUBMED    9353319
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1997 Nov 7. 272(45):28557-62."
COMMENT     Sequence Label: HA-NEDD8
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             96..122
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(423..441)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(424..441)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(461..478)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    467..691
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(824..843)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttatgtaccc atacgatgtt ccggattacg
      121 ctagcggatc catgctaatt aaagtgaaga cgctgaccgg aaaggagatt gagattgaca
      181 ttgaacctac agacaaggtg gagcgaatca aggagcgtgt ggaggagaaa gagggaatcc
      241 ccccacaaca gcagaggctc atctacagtg gcaagcagat gaatgatgag aagacagcag
      301 ctgattacaa gattttaggt ggttcagtcc ttcacctggt gttggctctg agaggaggat
      361 gagaattctg cagatatcca tcacactggc ggccgctcga gcatgcatct agagggccct
      421 attctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt
      481 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
      541 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
      601 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
      661 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
      721 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
      781 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
      841 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
      901 ttagg
//
