LOCUS       sequence-62514-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ChR2-Venus for neuronal excitation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Petreanu L, Huber D, Sobczyk A, Svoboda K
  TITLE     Channelrhodopsin-2-assisted circuit mapping of long-range callosal 
            projections.
  JOURNAL   Nat Neurosci. 2007 May . 10(5):663-8.
  PUBMED    17435752
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2007 May . 10(5):663-8."
COMMENT     Sequence Label: pCAGGS-ChR2-Venus
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(45..64)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          47..179
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(208..459)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(250..271)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     599..618
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     858..879
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattgt cgacacctgt ggagagaaag
       61 gcaaagtgga tgtcagtaag accaataggt gcctatcaga aacgcaagag tcttctctgt
      121 ctcgacaagc ccagtttcta ttggtctcct taaacctgtc ttgtaacctt gatacttacc
      181 tgcggcctcg agtccggggc cgctttactt gtacagctcg tccatgccga gagtgatccc
      241 ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct
      301 cagcttggac tggtagctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat
      361 gggggtgttc tgctggtagt ggtcggcgag ctgcacgccg ccgtcctcga tgttgtggcg
      421 gatcttgaag ttggccttga tgccgttctt ctgcttgtcg gcggtgatat agacgttgtg
      481 gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat
      541 gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt
      601 cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat
      661 ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcgggcga agcactgcag
      721 gccgtagccc agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca
      781 gatcagcttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct
      841 gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgg
//
