LOCUS       sequence-62515-e         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Gupta A, Hall VL, Kok FO, Shin M, McNulty JC, Lawson ND, Wolfe SA
  TITLE     Targeted Chromosomal Deletions and Inversions in Zebrafish.
  JOURNAL   Genome Res. 2013 Mar 11.
  PUBMED    23478401
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2013 Mar 11."
COMMENT     Sequence Label: pJDS71-GG-SHD
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..60)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(76..663)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 gtgatgatga ccggtacgcg tagaatcgag accgaggaga gggttaggga taggcttacc
       61 ttcgaagggc ccttaaaagt ttatctcgcc gttattaaat ttccgtctga cttcctctaa
      121 ggttaatgtg ccggctttaa tcatttctcc accaattaaa agctcttcta cactaagaac
      181 agctccatta caattagtga tatgatttaa tcgtgtaagc tgagctttgt agtttccttt
      241 aaagtgacca ctcacaaata aaaacttaaa ttccgttaca gaagatggat agactttcca
      301 ccattcatta gggttgatat gtttgtttcg tgtttgattt tcttcgacat atcgttgcat
      361 ttcatctgct tggccaattg gcagattata acctccgcta taagctttag tatccacgat
      421 cacaccgtaa tcaataggag atccgacagt ataaattgct ccgtccggtt tccttgatcc
      481 acccaaatgt ttacctctat atccataaac tttcataaaa aattccatta ccttcatttc
      541 aagaattcta tcctgagtgg aatttctggc aatttcaatt aattcaatat attcatgagg
      601 cacatatttc aatttatgac gaagttcaga tttcttctcc tccagttcac ttttgactag
      661 ttgggatccc gcgactcgat gggaagttct ctcgggaatc cgccggttgg ttcttttgat
      721 caatgcggga gcatgagg
//
