LOCUS       sequence-62528-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Liu DS, Phipps WS, Loh KH, Howarth M, Ting AY
  TITLE     Quantum Dot Targeting with Lipoic Acid Ligase and HaloTag for 
            Single-Molecule Imaging on Living Cells.
  JOURNAL   ACS Nano. 2012 Dec 21;6(12):11080-7. doi: 10.1021/nn304793z. Epub 
            2012 Dec 5.
  PUBMED    23181687
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/nn304793z";
            journalName: "ACS Nano"; date: "2012-12-21- 21"; volume: "6"; issue:
            "12"; pages: "11080-7"
COMMENT     Sequence Label: pCAG-AILR-LplA-ER
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(530..546)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(530..546)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(543..565)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    554..570
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(578..584,585..602,603..608))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    623..644
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggccgccaag ggcatcacca gcgtgagaag cagagtgacc aacctgaccg agctgctgcc
       61 cggcatcacc cacgagcagg tgtgcgaggc catcaccgag gccttcttcg cccactacgg
      121 cgagagagtg gaggccgaga tcatcagccc caacaagacc cccgacctgc ccaacttcgc
      181 cgagaccttc gccagacaga gcagctggga gtggaacttc ggccaggccc ccgccttcag
      241 ccacctgctg gacgagagat tcacctgggg cggcgtggag ctgcgcttcg acgtggagaa
      301 gggccacatc accagagccc aggtgttcac cgacagcctg aaccccgccc ccctggaggc
      361 cctggccggc agactgcagg gctgcctgta cagagccgac atgctgcagc aggagtgcga
      421 ggccctgctg gtggacttcc ccgagcagga gaaggagctg agagagctga gcgcctggat
      481 ggccggcgcc gtgagaaagg acgagctgta aaagcttggc gtaatcatgg tcatagctgt
      541 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
      601 agtgtaaagc ctggngtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
      661 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc
      721 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccc
//
