LOCUS       sequence-62530-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Gupta A, Hall VL, Kok FO, Shin M, McNulty JC, Lawson ND, Wolfe SA
  TITLE     Targeted Chromosomal Deletions and Inversions in Zebrafish.
  JOURNAL   Genome Res. 2013 Mar 11.
  PUBMED    23478401
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2013 Mar 11."
COMMENT     Sequence Label: pJDS74-GG-NNN
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..66)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(82..669)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 gtgatggtga tgatgaccgg tacgcgtaga atcgagaccg aggagagggt tagggatagg
       61 cttaccttcg aagggccctt aaaagtttat ctcgccgtta ttaaatttcc gtctgacttc
      121 ctctaaggtt aatgtgccgg ctttaatcat ttctccacca attaaaagct cttctacact
      181 aagaacagct ccattacaat tagtgatatg atttaatcgt gtaagctgag ctttgtagtt
      241 tcctttaaag tgaccactca caaataaaaa cttaaattcc gttacagaag atggatagac
      301 tttccaccat tcattagggt tgatatgttt gtttcgtgtt tgattttctt cgacatatcg
      361 ttgcatttca tctgcttggc caattggcag attataacct ccgctataag ctttagtatc
      421 cacgatcaca ccgtaatcaa taggagatcc gacagtataa attgctccgt ccggtttcct
      481 tgatccaccc aaatgtttac ctctatatcc ataaactttc ataaaaaatt ccattacctt
      541 catttcaaga attctatcct gagtggaatt tctggcaatt tcaattaatt caatatattc
      601 atgaggcaca tatttcaatt tatgacgaag ttcagatttc ttctcctcca gttcactttt
      661 gactagttgg gatcccgcga ctcgatggga agttctctcg ggaatccgcc ggttggttct
      721 tttgatcaat gcgggagcat gaggcagacc ctttttgact gcatcgagcg cgggtcgtcc
      781 accaagacat gccaacgcca ccagatggtc attcgttaac gcagccaacg cgggatcggg
      841 cctggacaat tgggct
//
