LOCUS       sequence-62532-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Gupta A, Hall VL, Kok FO, Shin M, McNulty JC, Lawson ND, Wolfe SA
  TITLE     Targeted Chromosomal Deletions and Inversions in Zebrafish.
  JOURNAL   Genome Res. 2013 Mar 11.
  PUBMED    23478401
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2013 Mar 11."
COMMENT     Sequence Label: pJDS78-GG-SNG
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             91..156
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             163..183
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagcaccatg gactacaaag accatgacgg tgattataaa
      121 gatcatgaca tcgattacaa ggatgacgat gacaagatgg cccccaagaa gaagaggaag
      181 gtgggcattc accgcggggt acctatggtg gacttgagga cactcggtta ttcgcaacag
      241 caacaggaga aaatcaagcc taaggtcagg agcaccgtcg cgcaacacca cgaggcgctt
      301 gtggggcatg gcttcactca tgcgcatatt gtcgcgcttt cacagcaccc tgcggcgctt
      361 gggacggtgg ctgtcaaata ccaagatatg attgcggccc tgcccgaagc cacgcacgag
      421 gcaattgtag gggtcggtaa acagtggtcg ggagcgcgag cacttgaggc gctgctgact
      481 gtggcgggtg agcttagggg gcctccgctc cagctcgaca ccgggcagct gctgaagatc
      541 gcgaagagag ggggagtaac agcggtagag gcagtgcacg cctggcgcaa tgcgctcacc
      601 ggggccccct tgcccctgga gacgattaat cccggtcgtc tccctatcgc ttctaatggg
      661 ggaggacggc cagccttgga gtccatcgta gcccaattgt ccaggcccga tcccgcgttg
      721 gctgcgttaa cgaatgacca tctggtggcg ttggcatgtc ttggtggacg acccgcgctc
      781 gatgcagtca aaaagggtct gcctcatgct cccgcattga tcaaaagaac caaccggcgg
      841 attccc
//
