LOCUS       sequence-62533-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  TITLE     Other Pak1 plasmids
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGEXTK-Pak1 70-117
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(251..273)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(418..436)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        504..608
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(827..846)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aatcggatct ggttccgcgt ggatctcgtc gtgcatctgt tggatccaaa gagcggccag
       61 agatttctct cccttcagat tttgaacaca caattcatgt cggttttgat gctgtcacag
      121 gggagtttac gggaatgcca gagcagtggg cccgcttgct tcagacatca aatatcacta
      181 agtcggagca gtgaattcat cgtgactgac tgacgatctg cctcgcgcgt ttcggtgatg
      241 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
      301 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
      361 cagccatgac ccagtcacgt agcgatagcg gagtgtataa ttcttgaaga cgaaagggcc
      421 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
      481 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
      541 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      601 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      661 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      721 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      781 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      841 tattatcccg tgttg
//
