LOCUS       sequence-62545-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Shigetomi E, Bushong EA, Haustein MD, Tong X, Jackson-Weaver O, 
            Kracun S, Xu J, Sofroniew MV, Ellisman MH, Khakh BS
  TITLE     Imaging calcium microdomains within entire astrocyte territories and
            endfeet with GCaMPs expressed using adeno-associated viruses.
  JOURNAL   J Gen Physiol. 2013 Apr 15.
  PUBMED    23589582
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gen 
            Physiol. 2013 Apr 15."
COMMENT     Sequence Label: pZac2.1 gfaABC1D-Lck-GCaMP3
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             102..119
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             123..155
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(159..167,168..182)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      189..198
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             210..266
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     479..500
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(606..627)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aagatgactg gatggagaac attgacgtgt gtgagaactg ccattatccc ccgccgaagc
       61 ttcgaattga tccaccggat ctcgccacca tgcggggttc tcatcatcat catcatcatg
      121 gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata
      181 aggatctcgc caccatggtc gactcatcac gtcgtaagtg gaataagaca ggtcacgcag
      241 tcagagctat aggtcggctc agctcactcg agaacgtcta tatcaaggcc gacaagcaga
      301 agaacggcat caaggcgaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc
      361 tcgcctacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
      421 accactacct gagcgtccag tccaaacttt cgaaagaccc caacgagaag cgcgatcaca
      481 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
      541 agggcggaac cggagggagc atggtgagca agggcgagga gctgttcacc ggggtggtgc
      601 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      661 gtgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      721 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
      781 gctaccccga ccacatgaag cagcacgact tcttcaagtc
//
